Cardiac safety of indacaterol in healthy subjects: a randomized, multidose, placebo- and positive-controlled, parallel-group thorough QT study
© Khindri et al; licensee BioMed Central Ltd. 2011
Received: 21 December 2010
Accepted: 26 May 2011
Published: 26 May 2011
Indacaterol is a novel once-daily ultra long-acting β2-agonist for the treatment of chronic obstructive pulmonary disease. It is known that β2-agonists, like other adrenergic compounds, can prolong the QT-interval. This thorough QT/QTc study (as per ICH E14 guideline) evaluated the effect of indacaterol on the QT interval in healthy subjects.
In this randomized, double-blind, parallel-group, placebo- and positive-controlled (open-label moxifloxacin) study, non-smoking healthy subjects (18-55 years, body mass index: 18.5-32.0 kg/m2) were randomized (4:4:2:4:1) to 14-day treatment with once-daily indacaterol (150 μg, 300 μg, or 600 μg), placebo, or placebo/moxifloxacin (double-blind 14-day treatment with placebo and a single open-label dose of 400 mg moxifloxacin on Day 14). The primary endpoint was the change from baseline on Day 14 in QTcF (QT interval corrected for heart rate using Fridericia's formula).
In total, 404 subjects were randomized to receive indacaterol (150 [n = 108], 300 [n = 108], 600 μg [n = 54]), placebo (n = 107), or placebo/moxifloxacin (n = 27); 388 subjects completed the study. Maximal time-matched mean (90% confidence intervals) treatment differences from placebo in QTcF change from baseline on Day 14 were 2.66 (0.55, 4.77), 2.98 (1.02, 4.93) and 3.34 (0.86, 5.82) ms for indacaterol 150 μg, 300 μg and 600 μg, respectively. Study sensitivity was confirmed with moxifloxacin demonstrating a significant maximal time-matched QTcF prolongation of 13.90 (10.58, 17.22) ms compared to placebo. All indacaterol doses were well tolerated.
Indacaterol, at doses up to 600 μg once daily (2-4 times the therapeutic dose) does not have any clinically relevant effect on the QT interval.
Indacaterol is a novel, inhaled, ultra long-acting β2-agonist (LABA)  that is approved in a number of countries, including throughout the European Union (EU), at doses of 150 and 300 μg once-daily (OD) for the maintenance bronchodilator treatment of airflow obstruction in adult patients with chronic obstructive pulmonary disease (COPD). It is a partial agonist at the human β2-adrenoceptor with a binding affinity similar to that of formoterol, and an intrinsic activity higher than that of salbutamol and salmeterol [2, 3]. The efficacy and safety of indacaterol in patients with COPD have been previously evaluated in number of studies, where once-daily indacaterol has demonstrated 24-h bronchodilation with a rapid onset of action [4–8].
The current International Conference on Harmonisation (ICH) of Technical Requirements for Registration of Pharmaceuticals for Human Use E14 Guidance  recommends that all new non-antiarrhythmic drugs that are systemically bioavailable should undergo a rigorous electrocardiographic evaluation as part of a controlled clinical study (that is a "thorough QT study"), to evaluate their potential QT liability. An abnormal QT-interval morphology is considered to be a surrogate biomarker for the proarrhythmic risk of non-antiarrhythmic drugs. Results from a meta-analysis of 33 randomized placebo-controlled trials (N = 6623) evaluating the arrhythmogenic potential of β-agonists  suggest that inhaled β2-agonists have a potential to increase heart rate and the incidence of ventricular arrhythmias in patients with obstructive airway disease. It is, therefore, especially important to conduct a thorough QT study as part of the development of an inhaled β2-agonist.
A thorough QT study is typically carried out in healthy volunteers as opposed to individuals at increased risk of arrhythmias. It is driven by a test of non-inferiority on the rate-corrected QT interval (the QTc interval) and is considered negative when the largest time-matched mean difference in the QTc interval between the study drug and placebo is less than or equal to (approximately) 5 ms, with the upper bound of the 95% one-sided confidence interval (CI) excluding 10 ms .
The present study, which, to the best of our knowledge, is the largest thorough QT study conducted thus far with any β2-agonist, was designed primarily to characterize the maximum mean QTc interval prolongation following treatment with three different doses of indacaterol in healthy individuals. Safety and tolerability and pharmacokinetics of indacaterol were also assessed.
This was a randomized, multiple-dose, placebo- and positive-controlled, parallel-group study conducted at a single clinical research center in the US. The study design was in compliance with the recommendation of the ICH E14 guideline  for thorough QT studies. The study was approved by the institutional review board (Independent Investigational Review Board; Sea View Research, Inc.) of the participating study center and was conducted in accordance with the ethical principles embodied in the Declaration of Helsinki (1989).
The study population consisted of healthy, nonsmoking male and female subjects aged between 18 and 55 years (inclusive), with a body mass index within the range of 18.5-32.0 kg/m2 and weight ≥50 kg at screening. Individuals with a history of or current ECG abnormalities (PR >240 ms; QRS complex >110 ms; QTc interval [Fridericia's; QTcF] >430 ms for males and >440 ms for females; or any significant morphological changes other than nonspecific T-wave changes) were excluded from the study. In addition, those with serum potassium <3.5 mmol/L or magnesium <0.8 mmol/L; recent blood donation or blood loss; or a history of diabetes mellitus, hyperthyroidism, impaired renal function, drug or alcohol abuse, or any significant illness within 2 weeks of dosing were also excluded. All subjects provided written informed consent prior to taking part in the study.
Study design and treatments
The study comprised a screening period (up to 21 days), a baseline visit (Day -1), a 14-day randomized treatment period (Days 1-14), and an end-of-study evaluation at 7 days after the last dose. At the start of the randomized treatment period, eligible subjects were randomized to one of the following five treatment groups in a 4:4:2:4:1 ratio: indacaterol 150 and 300 μg (therapeutic doses), indacaterol 600 μg (supratherapeutic dose), placebo, and placebo/moxifloxacin. Moxifloxacin, a fluoroquinolone antimicrobial agent, was used as a positive calibrator to determine the assay sensitivity in this study. This dose of 400 mg represents the daily therapeutic dose of moxifloxacin, single doses of which have been consistently associated with an increase in mean QT interval vs placebo in excess of 5 ms in healthy volunteers .
Indacaterol and matching placebo were administered once daily for 14 days via a single-dose dry powder inhaler (SDDPI) under double-blind conditions. Subjects randomized to the placebo/moxifloxacin arm received double-blind placebo via SDDPI for 14 days, with a single open-label oral dose of moxifloxacin 400 mg on Day 14.
Except for paracetamol and any medication needed to treat adverse events (AEs), no medication other than study drug was allowed from the start of screening until the completion of all evaluations in the study.
Serial 12-lead surface ECGs were obtained using digital ECG equipment (ELI 250™, Mortara Instrument Inc., Milwaukee, WI, USA) at baseline (Day -1) at the equivalent of predose and 1 and 12 h post-dose. During the treatment period, three ECG recordings were collected at each of the following times on Days 1 and 14: at predose, and at 10, 20, and 40 min, and 1, 2, 3, 4, 6, 12, and 24 h post-dose. All ECGs were recorded after subjects had been resting in the supine position for at least 15 min (or 8 min for the 10 min post-dose reading). The digital ECG recordings were transmitted electronically to a central facility for blinded manual interpretation and analysis. All ECG readers were blinded to all elements of the study, including treatment assignments and subject demographics.
Pharmacokinetic samples were taken to determine the relationship between indacaterol concentration and QTc interval. Serial blood samples were collected on Days 1 and 14 at predose and at 10, 20, and 40 min, and 1, 2, 3, 4, 6, 12, and 24 h post-dose. Serum was obtained from all blood samples by centrifugation and kept frozen at ≤ -20°C until analysis. Serum indacaterol was measured using a liquid chromatography/mass spectrometry/mass spectrometry method; the lower limit of quantification for indacaterol was 10 pg/mL. The maximum (peak) serum concentration (Cmax) and the area under the concentration-time curve from 0 to 24 h post-dose (AUC0-24h) were determined on Days 1 and 14, and were presented as the mean and standard deviation (SD).
Safety assessments included recording of AEs and serious adverse events (SAEs) with severity and relationship to study drug; collection of clinical laboratory data for urinalysis, hematology, and blood chemistry (including serum potassium and plasma glucose); and regular assessments of vital signs, ECGs, and body weight.
Sample size calculation and statistical analysis
The primary endpoint for the study was the QTcF interval (QT interval corrected for heart rate using Fridericia's formula, QT/RR0.33). This formula is recommended for investigating the effect of drugs that may affect pulse rate [9, 12, 13]. For each of three ECGs taken at each nominal time, the QT and RR intervals were measured from three consecutive QRST complexes from lead II. The mean of the triplicate ECG value was then calculated.
The primary objective of this study was to compare indacaterol 150, 300 and 600 μg with placebo in terms of the change in QTcF interval from baseline at each of the 10 post-dose time points on Day 14. Baseline QTcF interval was defined as the average of the pre-treatment measurements taken on Day -1 and the predose measurement on Day 1.
The sample size calculation was based on a residual standard deviation of 10.95 ms for the primary endpoint observed in a previous indacaterol study (data on file). Assuming a 5 ms difference between indacaterol 150 μg or 300 μg and placebo in terms of change from baseline in QTcF interval at the first four post-dose assessment time points on Day 14 and no difference at the next six time points, it was calculated that a sample size of 102 subjects was required in each treatment group to demonstrate that all upper one-sided 95% confidence intervals were below 10 ms.
For the comparison of indacaterol 600 μg versus placebo, 52 subjects in each treatment arm were required in order to show that all upper one-sided 95% confidence intervals were <20 ms. This calculation assumed an estimated difference of 13 ms between indacaterol 600 μg and placebo in terms of change from baseline in QTcF interval at the first four assessment time points and no difference between the treatments at the next six time points.
For the comparison between moxifloxacin and placebo, 26 subjects per treatment arm were required to conclude an assay sensitivity with 95% power based on the assumption that the difference between moxifloxacin and placebo for QTcF interval change from baseline was at least 5 ms and that this difference could be detected at least at 4 of the 10 post-dose assessment time points on Day 14. Assuming a dropout rate of approximately 10%, the number of subjects to be randomized was 116, 116, 58, 116, and 29, for indacaterol 150, 300, 600 μg, placebo, and placebo/moxifloxacin, respectively.
The absence of any meaningful effect of indacaterol 150 and 300 μg on cardiac repolarization was to be concluded if the upper limit of all the 90% two-sided CIs (equivalent to the upper limit of a 95% one-sided CI) for the mean difference in the change in QTcF interval from baseline between indacaterol (150 and 300 μg) and placebo at each time point was <10 ms. A similar analysis was performed for the supratherapeutic dose of indacaterol (600 μg) with the noninferiority margin set at a prespecified constant of 20 ms for the upper limit of the 90% two-sided CIs. Confidence intervals were calculated from pairwise determination of the pooled standard error and the 10th and 90th percentiles of the t-distribution with appropriate degrees of freedom.
The average QTcF intervals (average of all scheduled post-dose QTcF measurements up to 24 h post-dose) and the peak QTcF intervals (highest QTcF interval value observed over the period of 10 min to 24 h post-dose) were calculated for all treatment groups on both Days 1 and 14. In addition, the incidences of absolute QTcF interval values >450, >480, and >500 ms were summarized for each treatment group, as were the incidences of changes from baseline in QTcF interval >30 and >60 ms.
To determine the relationship between steady-state serum concentration of indacaterol and QTcF interval, change from baseline in QTcF interval was plotted against the corresponding indacaterol concentration for each time point on Day 14 (including all observations under placebo and all indacaterol doses). A linear relationship between the two was established by plotting a regression line, with the upper 95% simultaneous confidence band also presented.
Subject disposition, demographics, and baseline characteristics
The study was conducted between April (first subject enrolled) and August 2008 (last subject completed). A total of 404 subjects were randomized, receiving indacaterol 150 μg (n = 108), indacaterol 300 μg (n = 108), indacaterol 600 μg (n = 54), placebo (n = 107), or placebo/moxifloxacin (n = 27); 388 subjects (96%) completed the study (94%, 94%, 98%, 97% and 100% for indacaterol 150, 300 and 600 μg, placebo and placebo/moxifloxacin, respectively). The most common reason for premature discontinuation was loss to follow up (n = 6) followed by withdrawal of consent (n = 4), AEs (n = 3), protocol deviations (n = 2), and abnormal test results (n = 1).
Demographic and baseline characteristics of the study participants
N = 108
N = 108
N = 54
N = 107
N = 27
Male, n (%)
Female, n (%)
Age (yrs), mean (SD)
Body mass index (kg/m2), mean (SD)
Weight (kg), mean (SD)
QT interval data
Effect of indacaterol and moxifloxacin on QTcF interval
Difference versus placebo in the change in QTcF interval from baseline on Day 14
Time post- dose
Placebo/moxifloxacin vs placebo
150 μg vs placebo
300 μg vs placebo
600 μg vs placebo
Mean (90% confidence intervals), in ms
1.62 (-0.34, 3.57)
1.26 (-0.64, 3.16)
1.56 (-0.83, 3.94)
1.84 (-1.32, 5.01)
2.23 (0.34, 4.12)
2.24 (0.45, 4.03)
2.84 (0.72, 4.95)
3.74 (0.97, 6.51)
2.12 (0.21, 4.03)
1.54 (-0.41, 3.49)
1.40 (-0.96, 3.76)
8.25 (5.04, 11.47)
0.89 (-1.12, 2.89)
1.05 (-0.99, 3.09)
0.70 (-1.72, 3.12)
10.76 (7.60, 13.93)
2.66 (0.55, 4.77)
2.98 (1.02, 4.93)
2.18 (-0.25, 4.61)
13.90 (10.58, 17.22)
0.48 (-1.53, 2.50)
1.06 (-0.91, 3.03)
2.14 (-0.19, 4.48)
11.12 (8.08, 14.15)
0.36 (-1.82, 2.53)
0.29 (-1.76, 2.33)
1.17 (-1.26, 3.59)
11.91 (8.57, 15.24)
0.46 (-1.70, 2.62)
1.21 (-0.64, 3.05)
3.34 (0.86, 5.82)
9.25 (5.93, 12.57)
0.44 (-1.69, 2.57)
-1.24 (-3.26, 0.79)
-1.62 (-4.23, 0.98)
4.24 (0.82, 7.67)
1.88 (0.12, 3.64)
0.67 (-1.15, 2.49)
-0.08 (-2.27, 2.11)
7.77 (4.73, 10.82)
Moxifloxacin administration resulted in a clinically significant increase in QTcF, thereby establishing the assay sensitivity of the trial. The largest mean difference between placebo/moxifloxacin and placebo alone (13.90 ms) was observed at 2 h post-dose; the corresponding upper bound of the two-sided 90% CI (17.22 ms) exceeded the regulatory threshold at this timepoint and at several other timepoints (Table 2, Figure 1) .
Difference between indacaterol and placebo for average and peak QTcF on Days 1 and 14
Indacaterol 150 μg vs placebo
Indacaterol 300 μg vs placebo
Indacaterol 600 μg vs placebo
Least squares mean (90% confidence intervals)
Average QTcF (ms)
Peak QTcF (ms)
Two subjects, one each from the indacaterol 150 μg (20 min post-dose on Day 14) and 300 μg (10 min post-dose on Day 1) dose groups, demonstrated QTcF interval values exceeding 450 ms; no values exceeded 480 ms. There were no QTcF changes from baseline >60 ms. A total of ten changes >30 ms were recorded: five with indacaterol 150 μg (one subject had three values and two others had one value >30 ms each); three with placebo (three subjects with one value >30 ms each); and two with placebo/moxifloxacin (one subject with two values >30 ms).
Cmax and AUC0-24h of indacaterol after once-daily inhalation on Days 1 and 14
mean ± SD
mean ± SD
252.9 ± 120.84
1202.0 ± 553.93
537.2 ± 224.23
2639.4 ± 862.08
1043.8 ± 285.47
5279.1 ± 1155.28
438.6 ± 196.38
3881.7 ± 1545.39
858.6 ± 264.16
8137.0 ± 2388.41
1656.7 ± 540.76
15084.6 ± 3428.15
Concentration-QTcF interval relationship
The overall incidence of AEs was 47.2% (51/108), 54.6% (59/108), and 61.1% (33/54) with indacaterol 150 μg, 300 μg, and 600 μg, respectively, as compared with 47.7% (51/107) with placebo, and 51.9% (14/27) with placebo/moxifloxacin. The most common AE was contact dermatitis (indacaterol 150 μg: n = 31 [28.7%]; indacaterol 300 μg: n = 36 [33.3%]; indacaterol 600 μg: n = 21 [38.9%]; placebo: n = 30 [28.0%]; placebo/moxifloxacin: n = 6 [22.2%]) attributed to the repeated application and removal of adhesive ECG electrodes. AEs were mostly mild in severity and in most cases were not suspected to be study drug related. Two subjects experienced SAEs, one receiving indacaterol 300 μg (chest pain) and one receiving placebo (acute depression). Neither event was suspected to be study drug related, although both subjects were withdrawn from the study. No death was reported during the study. There were no clinically notable serum potassium or blood glucose values or any abnormalities pertaining to heart rate or other vital signs in any group.
In this study, indacaterol 150 μg, 300 μg, and 600 μg, administered once-daily for 14 days resulted in a maximum mean prolongation in QTcF of less than 5 ms versus placebo, with the upper limit of all two-sided 90% CIs being below 10 ms. Therefore, according to the ICH E14 guidelines , the present thorough QT study can be deemed negative, indicating that indacaterol has no QT effects of concern across the investigated dose range.
In terms of dose-effect relationship, the highest dose of indacaterol (600 μg) resulted in the largest QT interval prolongation on Day 14 (3.34 ms at 6 h post-dose); however, the respective 90% CIs for all indacaterol doses largely overlapped with one another suggesting the absence of any dose-QTcF relationship. The lack of dose-dependency in the investigated dose range further supported by the mean QTcF changes from baseline on Day 14, which showed similar, small (<5 ms) and transient increases in QTcF interval in each indacaterol dose group compared with placebo. Further, on Day 14 there were only marginal and non-significant changes in average and peak QTcF intervals with all three doses of indacaterol, with the concentration-QTcF interval analysis indicating that a prolongation above 10 ms is not expected at doses of indacaterol resulting in steady-state Cmax values up to 3000 pg/mL. This is particularly relevant, given that according to the prescribing information for formoterol and salmeterol, at high doses these other β2-agonists are associated with QT interval prolongation. Since this study did not include any other LABAs, one cannot draw any direct conclusions from the results about the relative cardiovascular safety of indacaterol, salmeterol and formoterol. Such a comparison is of interest, given that these three LABAs have different intrinsic efficacies . However a 12-month study in which patients with COPD were randomized to receive indacaterol 300 μg or 600 μg, formoterol or placebo, showed that all four treatments had similar good overall safety profiles . Furthermore, given that the current study recruited healthy volunteers (in accordance with ICH E14 guidelines), the earlier 12-month study is useful in that it also provides evidence of cardiovascular safety in patients with COPD and a range of comorbid conditions (including hypertension and Type II diabetes mellitus). In addition, in a recent post-hoc analysis of the indacaterol trial database, indacaterol did not increase the risk of cerebrovascular or cardiovascular adverse events compared with placebo, formoterol or salmeterol .
In accordance with the ICH E14 guidelines, moxifloxacin was included in the study as a positive control, and administration of a single 400 mg dose resulted in a clinically significant increase in QTcF interval, thereby establishing the assay sensitivity of the trial. QTc interval prolongation with moxifloxacin in this trial was similar to that reported previously .
The indacaterol doses administered in this study (150 μg, 300 μg, and 600 μg) were selected based on a dose finding study which used an adaptive seamless study design , and which identified the 150 and 300 μg doses for further development. These two doses were subsequently approved in a number of countries, including throughout the European Union, for maintenance treatment of COPD. Previous studies have demonstrated that patient age, weight and ethnicity have no clinically relevant effect on indacaterol pharmacokinetics (Cmax and AUC; data on file). Further, metabolic drug interactions raise the systemic exposure of indacaterol by a maximum of two-fold (data on file). In this context, the absence of prolongation of QTcF above 10 ms with the 600 μg dose (i.e., two- to four-fold higher than the approved EU doses) is a relevant finding for the safety profile of indacaterol.
Notably, in the current study no change from baseline in QTcF interval of >60 ms was observed with any of the indacaterol doses, and nor were any QTcF interval values >480 ms observed. These results also demonstrated that indacaterol did not prolong QTc interval and further suggests that there were no outliers in terms of QTc interval prolongation. Indeed in a previous study in patients with mild or moderate COPD, even at single doses as high as 3000 μg indacaterol had minimal effects on the QTc interval .
The results of this thorough QT study carried out in over 400 healthy subjects indicate that indacaterol, at doses up to 600 μg once daily (2-4 times the therapeutic dose) does not have any clinically relevant effect on the QT interval.
The authors thank the healthy volunteers who took part and the staff at the participating clinical centre. The authors acknowledge Shubhro Kamal Ghosh, professional medical writer (Novartis) and David Young (Novartis) for assistance in preparation of this manuscript.
- Cazzola M, Maters MG, Lötval J: Ultra long-acting β2-agonists in development for asthma and chronic obstructive pulmonary disease. Expert Opin Investig Drugs. 2005, 14: 775-783. 10.1517/135437184.108.40.2065.View ArticlePubMedGoogle Scholar
- Roig J, Hernando R, Ramon Mora R: Indacaterol, A Novel once daily inhaled beta2-adrenoreceptor agonist. Open Respir Med J. 2009, 3: 27-30. 10.2174/1874306400903010027.View ArticlePubMedPubMed CentralGoogle Scholar
- Battram C, Charlton SJ, Cuenoud B, Dowling MR, Fairhurst RA, Farr D, Fozard JR, Leighton-Davies JR, Lewis CA, McEvoy L, Turner RJ, Trifilieff A: In vitro and in vivo pharmacological characterization of 5-[(R)-2-(5,6-diethyl-indan-2-ylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quino lin-2-one (indacaterol), a novel inhaled beta(2) adrenoceptor agonist with a 24-h duration of action. J Pharmacol Exp Ther. 2006, 317: 762-770. 10.1124/jpet.105.098251.View ArticlePubMedGoogle Scholar
- Beier J, Chanez P, Martinot JB, Schreurs AJ, Tkacova R, Bao W, Jack D, Higgins M: Safety, tolerability and efficacy of indacaterol, a novel once-daily beta(2)-agonist, in patients with COPD: a 28-day randomised, placebo controlled clinical trial. Pulm Pharmacol Ther. 2007, 20: 740-749. 10.1016/j.pupt.2006.09.001.View ArticlePubMedGoogle Scholar
- Rennard S, Bantje T, Centanni S, Chanez P, Chuchalin A, D'Urzo A, Kornmann O, Perry S, Jack D, Owen R, Higgins M: A dose-ranging study of indacaterol in obstructive airways disease, with a tiotropium comparison. Respir Med. 2008, 102: 1033-1044. 10.1016/j.rmed.2008.02.001.View ArticlePubMedGoogle Scholar
- Bauwens O, Ninane V, Van de Maele B, Firth R, Dong F, Owen R, Higgins M: 24-hour bronchodilator efficacy of single doses of indacaterol in subjects with COPD: comparison with placebo and formoterol. Curr Med Res Opin. 2009, 25: 463-470. 10.1185/03007990802675096.View ArticlePubMedGoogle Scholar
- Donohue JF, Fogarty C, Lötvall J, Mahler DA, Worth H, Yorganciolu A, Iqbal A, Swales J, Owen R, Higgins M, Kramer B, for the INHANCE study investigators: Once-daily Bronchodilators for chronic obstructive pulmonary disease: indacaterol versus tiotropium. Am J Respir Crit Care Med. 2010, 182: 155-162. 10.1164/rccm.200910-1500OC.View ArticlePubMedGoogle Scholar
- Dahl R, Chung KF, Buhl R, Magnussen H, Nonikov V, Jack D, Bleasdale P, Owen R, Higgins M, Kramer B, on behalf of the INVOLVE (indacaterol: value in COPD pulmonary disease: longer term validation of efficacy and safety) study investigators: Efficacy of a new once-daily LABA, indacaterol, versus the twice-daily LABA, formoterol, in COPD. Thorax. 2010, 65: 473-479. 10.1136/thx.2009.125435.View ArticlePubMedGoogle Scholar
- US Food and Drug administration: International Conference on Harmonization (ICH) guidance documents: E14 clinical evaluation of QT/QTc interval prolongation and proarrhythmic potential for non-antiarrhythmic drugs. 2005Google Scholar
- Salpeter SR, Thomas TM, Salpeter EE: Cardiovascular effects of β-agonists in patients with asthma and COPD. Chest. 2004, 125: 2309-2321. 10.1378/chest.125.6.2309.View ArticlePubMedGoogle Scholar
- Démolis JL, Kubitza D, Tennezé L, Funck-Brentano C: Effect of a single oral dose of moxifloxacin (400 mg and 800 mg) on ventricular repolarization in healthy subjects. Clin Pharmacol Ther. 2000, 68: 658-666. 10.1067/mcp.2000.111482.View ArticlePubMedGoogle Scholar
- Morganroth J: Cardiac repolarization and the safety of new drugs defined by electrocardiography. Clin Pharmacol Ther. 2007, 81: 108-113. 10.1038/sj.clpt.6100010.View ArticlePubMedGoogle Scholar
- Milic M, Bao X, Rizos D, Liu F, Ziegler MG: Literature review and pilot studies of the effect of QT correction formulas on reported beta2-agonist-induced QTc prolongation. Clin Ther. 2006, 28: 582-590. 10.1016/j.clinthera.2006.04.010.View ArticlePubMedGoogle Scholar
- Naline E, Trifilieff A, Fairhurst RA, Advenier C, Molimard M: Effect of indacaterol, a novel long-acting β2-agonist, on isolated human bronchi. Eur Respir J. 2007, 29: 575-581. 10.1183/09031936.00032806.View ArticlePubMedGoogle Scholar
- Worth H, Chung KF, Felser JM, Hu H, Rueegg P: Cardio- and cerebrovascular safety of indacaterol vs formoterol, salmeterol, tiotropium and placebo in COPD. Respir Med. 2011, 105: 571-579. 10.1016/j.rmed.2010.11.027.View ArticlePubMedGoogle Scholar
- Barnes PJ, Pocock SJ, Magnussen H, Iqbal A, Kramer B, Higgins M, Lawrence D: Integrating indacaterol dose selection in a clinical study in COPD using and adaptive seamless design. Pulm Pharmacol Ther. 2010, 23: 165-171. 10.1016/j.pupt.2010.01.003.View ArticlePubMedGoogle Scholar
- Pascoe S, Reynolds C, Pleskow W, Perry S, Hmissi A, Kaiser G, Brookman L: Safety, tolerability and pharmacokinetics of single escalating doses of indacaterol, a once-daily β2-agonist bronchodilator, in subjects with COPD. Int J Clin Pharmacol Ther. 2011, 49: 153-161.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2466/11/31/prepub