Nutrition of preterm infants in relation to bronchopulmonary dysplasia
© Wemhöner et al; licensee BioMed Central Ltd. 2011
Received: 26 August 2010
Accepted: 3 February 2011
Published: 3 February 2011
The pathogenesis of bronchopulmonary dysplasia (BPD) is multifactorial. In addition to prenatal inflammation, postnatal malnutrition also affects lung development.
A retrospective study was performed to analyse during the first two weeks of life the total, enteral and parenteral nutrition of premature infants (<31 weeks, birth weight ≤1500 g) born between 08/04 and 12/06.
Ninety-five premature infants were analysed: 26 with BPD (27 ± 1 weeks) and 69 without BPD (28 ± 1 weeks). There was no statistical significant difference in the total intake of fluids, calories, glucose or protein and weight gain per day in both groups. The risk of developing BPD was slightly increased in infants with cumulative caloric intake below the minimal requirement of 1230 kcal/kg and a cumulative protein intake below 43.5 g/kg. Furthermore, the risk of developing BPD was significantly higher when infants had a cumulative fluid intake above the recommended 1840 ml/kg. In infants who developed BPD, the enteral nutrition was significantly lower than in non-BPD infants [456 ml/kg (IQR 744, 235) vs. 685 (IQR 987, 511)]. Infants who did not develop BPD reached 50% of total enteral feeding significantly faster [9.6 days vs. 11.5].
Preterm infants developing BPD received less enteral feeding, even though it was well compensated by the parenteral nutrient supply. Data suggest that a critical minimal amount of enteral feeding is required to prevent development of BPD; however, a large prospective clinical study is needed to prove this assumption.
Bronchopulmonary dysplasia (BPD) is a chronic pulmonary disease that affects preterm infants. In the past, BPD was mainly caused by ventilatory injury and affected about 30% of preterm infants with birth weights below 1000 grams . Gentler ventilation techniques, antenatal steroids and surfactant treatment have reduced the incidence of lung injury. Despite this progress, the incidence of BPD is not decreasing, but the picture of BPD is changing. The so called "new BPD" is a lung development problem that involves impairment of alveolarisation which results in large, simplified alveolar structures, dysmorphic capillary configuration and variable interstitial cellularity and/or fibroproliferation .
Several factors are considered responsible for altering lung development and may subsequently support the development of BPD. In addition to prenatal inflammation, nutrition plays an important role in normal lung development and maturation [2, 3] Nutrition has a direct effect on the developing lung because it can modulate lung structure. General under-nutrition in humans leads to lung emphysema [4, 5]. In rats, caloric restriction reduces the alveolar number by 55% and the alveolar surface area by 25% . Seventy two hours after re-feeding, however, rat lungs are re-modelled with normal alveolar numbers and surface areas .
Sufficient nutrition is often difficult to achieve in preterm infants. Due to various problems associated with immaturity, extremely preterm infants receive only minimal enteral nutrition during the first weeks of life and require supplemental parenteral nutrition. However, there is an ongoing debate concerning the required amount of protein, carbohydrates and calories . A sufficient amount of protein and calories seems to be necessary for organ growth; thus, a low protein or caloric intake could impair lung development, resulting in BPD.
The present study was performed to test the hypothesis that nutrition intake during the first 2 weeks of life is lower in infants who subsequently develop BPD. Furthermore, the effects of protein, calorie and carbohydrate intake and the percentage of enteral nutrition during the first two weeks on the subsequent development of BPD were studied. The charts for all preterm infants under 31 weeks and below 1500 g who were born in our unit during a 17-month period were analysed.
The observational cohort study was performed in the neonatal intensive care unit (NICU) of a tertiary centre at the Department of Pediatrics, Neonatology at the University Hospital Innsbruck, Austria. The medical ethics committee of the Medical University Innsbruck approved the study.
Data were obtained from all preterm infants born between August 2004 and December 2006. All infants with birth weights below 1500 g and gestational ages below 31 weeks who were treated in the NICU during that period were included in the study. Infants were excluded from the analysis for the following reasons: death within the first week of life, transfer to another hospital and missing data.
The following demographic variables were obtained for all preterm infants born during the study period: birth weight, gestational age, mode of delivery, singleton pregnancy, 5 minute APGAR score and completed courses of antenatal steroids given.
The main outcome parameters were the cumulative amount of protein, calorie, carbohydrate and enteral feeding intake at 14 days of life. Parameters of total nutrition were obtained daily, and parameters of neonatal growth (weight, head circumference and length) were obtained daily up to day 14 and were then obtained at day 28 and 36 weeks postmenstrual age.
The following morbidity parameters were collected: Respiratory Distress Syndrome (RDS) incidence, surfactant application after intubation, surfactant application and the presence of an open ductus arteriosus as determined by echocardiography.
The following discharge parameters were obtained: body weight and length, head circumference and length of stay in hospital.
Definition of bronchopulmonary dysplasia
The aim of the study was to compare nutritional data during the first 14 days of life in infants who later developed BPD with data of non-BPD infants. BPD was defined as treatment with oxygen >21% for at least 28 days on 36 week postmenstrual age or discharge to home, whichever comes first as described elsewhere .
Enteral nutritional regime
During the study period, the feeding regime was not changed. Preterm infants were fed according to a local protocol that was adapted by the attending neonatologist to meet the actual requirements of the individual patient [10, 11]. According to NICU routine, oral feeding is started with either 0.5 [birth weight (b.w.) ≤ 750 g] or 1 ml (b.w. > 751 g) mother milk or donor breast milk as soon as possible after birth. Subsequently, the daily feeding volume was increased by about 10 ml/kg body weight. The volume was neither increased nor decreased if the attending staff noticed "feeding intolerance". Preterm infants were either fed with their mother's milk or donor breast milk. Milk fortification was usually performed if a feeding volume of more than 100 ml/kg*d was achieved.
Parenteral nutritional regime
Enteral nutrition was supplemented with parenteral nutrition if needed. Actual requirements were calculated daily with software that calculates fluid, calories, protein and glucose requirements for enteral and parenteral nutrition and lipids for parenteral application. The required parenteral nutrition was gradually tapered as enteral feeding volumes increased and was generally stopped if a feeding volume of 130 to 140 ml/kg of fortified milk was achieved . The protocol for parenteral nutrition started with a total of 70-80 ml/kg/d fluid on the first day of life . The fluid intake was increased by 10-20 ml/kg/day until 150 ml/kg/d was achieved. Protein administration was started during the first day of life with 1 g/kg/day and was increased by 0.5 g/kg per day until 3 g/kg is achieved. On the third day, intravenous lipid administration was started with 0.5 to 1 g/kg/d and was advanced in increments of 0.5 to 1 g/kg/d until 3 g/kg/d was achieved.
It is assumed that a "minimal nutritional requirement" is needed for undisturbed growth and that problems occur only below that critical threshold. Therefore, minimal requirements during the first two weeks were calculated according to recent recommendations [13, 14] protein 43.5 g/kg, calories 1110-1210 kcal/kg and carbohydrates 187-213 g/kg. Because volume overload is associated with the subsequent development of BPD, a "maximal" total fluid intake during the first two weeks of life was calculated at 1620-1800 ml/kg. The relative risk of developing BPD was calculated if the minimal requirements were not achieved.
Continuous data, which is normally distributed, is presented as mean/SD; for skewed data, medians with interquartile ranges are shown. Statistical analyses of outcome were conducted in order to compare data for BPD and non-BPD infants. Univariate comparisons of proteins, calories, carbohydrates and total fluid were performed using the non-parametric Mann-Whitney U-Test. All reported p-values are two-sided with a level of significance set at 0.05. All analyses were performed using SPSS 15.0 (Chicago, Illinois).
Perinatal data of the study group
Perinatal data Perinatal data for infants in BPD and non-BPD (NBPD) group.
Gestational age (weeks)
27 ± 1*
28 ± 1
Birth weight (gram)
965 ± 255*
1170 ± 246
35 ± 3.2*
37 ± 2.5
Head circumference (cm)
24.7 ± 2.1*
26 ± 1.6
Apgar score at 5 minutes
7.8 ± 1
7.9 ± 1
Small for gestational age
Completed courses of antenatal steroids
*p < 0.02 vs. NBPD
Body composition Data for the study population at the 14th day of life and at 36 weeks post-conception.
1015 ± 254*
1190 ± 241
1883 ± 286*
2038 ± 286
36.8 ± 3.3*
38.5 ± 2.6
42 ± 2*
43 ± 2
Head circumference (cm)
25 ± 2*
26.7 ± 1.7
30 ± 1.5*
31.4 ± 1.5
*p < 0.01 vs. NBPD
Cumulative nutrition management
Nutrient intake Total nutrient intake during the first two weeks of life.
Total fluid [ml/kg]
1864 (1792, 1929)
1860 (1813, 1904)
Total calories [kcal/kg]
1089 (966, 1190)
1154 (1081, 1221)
Total protein [g/kg]
46 (41, 49)
45 (41, 49)
Total carbohydrates [g/kg]
100 (187, 103)
102 (96, 108)
The difference in the total caloric intake during the first two weeks of life was not statistically significant between infants with and without subsequent BPD (table 3). The risk of developing BPD was slightly (but not significantly) higher if the caloric intake during the first two weeks of life was below a minimal requirement of 1230 kcal/kg (RR 1.92, 95% CI 0.6, 5.7).
The total intake of amino acids during the first two weeks of life was not significantly different between the two groups (table 3); however, there were large variations between infants. Again, the risk of developing BPD was (not significantly) higher if the protein intake was below the minimal requirement of 43.5 g/kg (RR 1.2, 95% CI 0.6, 2.4). Finally, the intake of carbohydrates during the first two weeks of life was not significantly different between the two groups (table 3).
Clinical outcome and other aspects of preterm morbidity
Analysis of data on postnatal respiratory morbidity revealed that significantly more infants received surfactant in the BPD group (80 vs. 52%, p < 0.01), whereas the incidence of RDS was similar in both groups (100 vs. 94%). There was a higher rate of ROP in the BPD group (57 vs. 21%, p = 0.001) and IVH (30 vs. 7% p = 0.004). Furthermore, the incidence of a persistent ductus arteriosus (42%), need for treatment ductus arteriosus (38%) and NEC > stage II (0%) were the same in both groups.
Finally, the length of stay in the hospital was significantly longer for infants with BPD than for infants who did not develop BPD (96 vs. 60 days, p < 0.01), respectively.
The present study tested the hypothesis that infants who develop BPD have lower caloric or protein intake during the first two weeks of life than infants without BPD. However, data from the current study do not support this assumption; the total intake of fluids, calories, amino acids, carbohydrates and weight gain per day was similar in infants with and without BPD. However, infants who developed BPD had a significantly lower amount of enteral nutrition during the first two weeks of life. Furthermore, the present study supports previous reports of the importance of fluid restriction during the first weeks of life to prevent the development of BPD [15, 16]. Whereas the median fluid intake was similar in both groups of the present study, infants who received more than the recommended amount of fluid had a significantly higher risk of developing BPD. In contrast to other studies [17, 18] we did not find any significant differences in calories and fat intake in the first 14 days of life. The reasons therefore might be a greater number of patients and a longer observational period in those studies.
Data from the present study are of great clinical interest because the impact of nutrition on BPD development has been discussed for a long time. Sosenko et al hypothesized that general under-nutrition, specifically insufficient protein intake, may increase the vulnerability of a preterm infant to oxidant-induced lung injury and the development of "old" BPD . Furthermore, nutrition plays an important role in lung growth and development. The "new BPD" is characterised by a rarefaction of alveolar structures, which could be partially explained by insufficient protein or caloric intake during the postnatal period. According to the present study, though, not all infants who developed BPD had low caloric intake. If intake of calories or protein was below the minimal requirement, infants were more likely to develop BPD. However, the values did not reach statistical significance due to the small number of patients in this study.
One of the major findings of the present study is the lower enteral intake during the first two weeks in infants who developed BPD. Infants with a greater illness severity may be at a higher risk of BPD, and may also have a greater magnitude of feeding intolerance or a reluctance on the part of the clinician to aggressively increase enteral feeds. It could be assumed that parenteral nutrition alone is insufficient to meet the nutritional needs of preterm infants [20, 21]. Furthermore, enteral nutrition could also have some beneficial effects for lung development. However, previous studies on enteral nutrition did not find a similar association between low enteral intake and subsequent development of BPD . While this study is of clinical interest, some limitations should be discussed. First of all, the data were obtained retrospectively. It was the aim of the study to show whether infants who develop BPD received fewer calories and protein despite the very strict guidelines on nutrition in our neonatal intensive care unit. Thus, it was of interest to see a large variation between infants. A second limitation is the small number of infants, which may have caused type II errors. Over a long study period of more than two years, changes in management are likely and, thus, interpretation of the data would be more difficult. However, the present study provides a sound base for a prospective study on this subject.
In conclusion, the present study shows that infants who developed BPD received less enteral feeding during the first two weeks of life, which was well compensated by the parenteral nutrient supply. It seems that a critical amount of protein and caloric intake is required to prevent the development of BPD; however, a large prospective randomized trial is needed to prove this assumption.
- Stevenson DK, Wright LL, Lemons JA, Oh W, Korones SB, Papile LA, et al: Very low birth weight outcomes of the National Institute of Child Health and Human Development Neonatal Research Network, January 1993 through December 1994. Am J Obstet Gynecol. 1998, 179: 1632-1639. 10.1016/S0002-9378(98)70037-7.View ArticlePubMedGoogle Scholar
- Coalson JJ: Pathology of new bronchopulmonary dysplasia. Semin Neonatol. 2003, 8: 73-81. 10.1016/S1084-2756(02)00193-8.View ArticlePubMedGoogle Scholar
- Jobe AH: Let's feed the preterm lung. J Pediatr (Rio J). 2006, 82: 165-166. 10.2223/JPED.1481.Google Scholar
- Coxson HO, Chan IH, Mayo JR, Hlynsky J, Nakano Y, Birmingham CL: Early emphysema in patients with anorexia nervosa. Am J Respir Crit Care Med. 2004, 170: 748-752. 10.1164/rccm.200405-651OC.View ArticlePubMedGoogle Scholar
- Massaro D, Massaro GD: Hunger disease and pulmonary alveoli. Am J Respir Crit Care Med. 2004, 170: 723-724. 10.1164/rccm.2408002.View ArticlePubMedGoogle Scholar
- Massaro GD, Radaeva S, Clerch LB, Massaro D: Lung alveoli: endogenous programmed destruction and regeneration. Am J Physiol Lung Cell Mol Physiol. 2002, 283: L305-L309.View ArticlePubMedGoogle Scholar
- Massaro D, Massaro GD, Baras A, Hoffman EP, Clerch LB: Calorie-related rapid onset of alveolar loss, regeneration, and changes in mouse lung gene expression. Am J Physiol Lung Cell Mol Physiol. 2004, 286: L896-L906. 10.1152/ajplung.00333.2003.View ArticlePubMedGoogle Scholar
- Atkinson SA: Special nutritional needs of infants for prevention of and recovery from bronchopulmonary dysplasia. J Nutr. 2001, 131: 942S-946S.PubMedGoogle Scholar
- Jobe A, Bancalari E: Bronchopulmonary dysplasia. Am J Respir Crit Care Med. 2001, 163: 1723-1729.View ArticlePubMedGoogle Scholar
- Kuzma-O'Reilly B, Duenas ML, Greecher C, Kimberlin L, Mujsce D, Miller D, et al: Evaluation, development, and implementation of potentially better practices in neonatal intensive care nutrition. Pediatrics. 2003, 111: e461-e470.View ArticlePubMedGoogle Scholar
- Pauls J, Bauer K, Versmold H: Postnatal body weight curves for infants below 1000 g birth weight receiving early enteral and parenteral nutrition. Eur J Pediatr. 1998, 157: 416-421. 10.1007/s004310050842.View ArticlePubMedGoogle Scholar
- Caple J, Armentrout D, Huseby V, Halbardier B, Garcia JG, Sparks JW, et al: Randomized, controlled trial of slow versus rapid feeding volume advancement in preterm infants. Pediatrics. 2004, 114: 1597-1600. 10.1542/peds.2004-1232.View ArticlePubMedGoogle Scholar
- Biniwale MA, Ehrenkranz RA: The role of nutrition in the prevention and management of bronchopulmonary dysplasia. Semin Perinatol. 2006, 30: 200-208. 10.1053/j.semperi.2006.05.007.View ArticlePubMedGoogle Scholar
- Thureen PJ: Early aggressive nutrition in very preterm infants. Nestle Nutr Workshop Ser Pediatr Program. 2007, 59: 193-204.PubMedGoogle Scholar
- Oh W, Poindexter BB, Perritt R, Lemons JA, Bauer CR, Ehrenkranz RA, et al: Association between fluid intake and weight loss during the first ten days of life and risk of bronchopulmonary dysplasia in extremely low birth weight infants. J Pediatr. 2005, 147: 786-790. 10.1016/j.jpeds.2005.06.039.View ArticlePubMedGoogle Scholar
- Mataloun MM, Leone CR, Mascaretti RS, Dohlnikoff M, Rebello CM: Effect of postnatal malnutrition on hyperoxia-induced newborn lung development. Braz J Med Biol Res. 2009, 42: 606-613. 10.1590/S0100-879X2009000700004.View ArticlePubMedGoogle Scholar
- Akram KM, Kuzma-O'Reilly B, Brodsky NL, Bhandari V: Site-specific characteristics of infants developing bronchopulmonary dysplasia. J Perinatol. 2006, 26: 428-435. 10.1038/sj.jp.7211538.View ArticleGoogle Scholar
- Kurzner SI, Garg M, Bautista DB, Bader D, Merritt RJ, Warburton D, et al: Growth failure in infants with bronchopulmonary dysplasia: nutrition and elevated resting metabolic expenditure. Pediatrics. 1988, 81: 379-384.PubMedGoogle Scholar
- Frank L, Sosenko IR: Undernutrition as a major contributing factor in the pathogenesis of bronchopulmonary dysplasia. Am Rev Respir Dis. 1988, 138: 725-729.View ArticlePubMedGoogle Scholar
- Cooke RW: Factors associated with chronic lung disease in preterm infants. Arch Dis Child. 1991, 66: 776-779. 10.1136/adc.66.7_Spec_No.776.View ArticlePubMedPubMed CentralGoogle Scholar
- Hammerman C, Aramburo MJ: Decreased lipid intake reduces morbidity in sick premature neonates. J Pediatr. 1988, 113: 1083-1088. 10.1016/S0022-3476(88)80587-0.View ArticlePubMedGoogle Scholar
- Ehrenkranz RA: Early, aggressive nutritional management for very low birth weight infants: what is the evidence?. Semin Perinatol. 2007, 31: 48-55. 10.1053/j.semperi.2007.02.001.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2466/11/7/prepub