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Table 2 Effect of insulin on the inflammatory cells infiltration into the airways and lung parenchyma

From: Insulin modulates cytokine release and selectin expression in the early phase of allergic airway inflammation in diabetic rats

 

Control

(non-diabetic)

Diabetic

Diabetic

+insulin

BALF (Cells × 106)

   

Mononuclears

14.28 ± 0.58

7.11 ± 0.18 *

13.08 ± 0.74 †

Neutrophils

9.63 ± 0.43

0.94 ± 0.11 *

9.51 ± 0.58 †

Eosinophils

0.04 ± 0.02

0.02 ± 0.01

0.03 ± 0.01

Perivascular (Cells/10 4 μm 2 )

   

Mononuclears

74.54 ± 2.65

56.75 ± 1.72 *

66.18 ± 2.15 †

Neutrophils

26.97 ± 1.56

15.58 ± 1.46 *

33.13 ± 3.75 †

Eosinophils

0.40 ± 0.16

0.50 ± 0.15

0.35 ± 0.13

Peribronchiolar (Cells/10 4 μm 2 )

   

Mononuclears

75.38 ± 2.90

56.30 ± 1.79 *

67.50 ± 3.10 †

Neutrophils

27.63 ± 2.44

18.08 ± 1.79 *

32.12 ± 3.23 †

Eosinophils

0.46 ± 0.24

0.25 ± 0.11

0.35 ± 0.17

  1. Rats were rendered diabetic by the injection of alloxan (42 mg/Kg, i.v.) 10 days before sensitization. In both non-diabetic and diabetic rats, active sensitization against ovalbumin (OA) was performed 14 days before OA intratracheal instillation. Insulin (NPH, 4 IU/rat, s.c.) was administered 2 hours before OA challenge. Bronchoalveolar lavage fluid (BALF) was performed 6 hours thereafter (9 animals in each group). Morphometric analysis was made in the stained slides of lung tissue. Determinations were made in 3 randomly selected fields for the presence of cells in the perivascular (4 vessels/sample) and peribronchiolar (4 bronchi/sample) tissue (3 animals in each group). Values are means ± SEM. *P < 0.001 comparing diabetic with control (non-diabetic) rats. † P < 0.001 comparing insulin-treated vs non-treated diabetic rats.
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BMC Pulmonary Medicine

ISSN: 1471-2466

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