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Table 2 Components necessary to induce SP-A self-agglutination under the assay conditions used

From: Assessment of Surfactant Protein A (SP-A) dependent agglutination

Condition Reagent Agglutination
  SP-A SP-D Goat-anti-human-SP-A antibody Anti-goat antibody  
1 + - + + +
2 - + + + -
3 + - - + -
4 + - + - -
5 - - + + -
  1. The table shows five different conditions of the bead-assay. The beads were incubated alternatively with anti-goat antibody, goat-anti-human antibody, SP-A (1 ng recombinant human standard, final concentration 100 ng/ml) or SP-D (1 ng recombinant human standard, final concentration 100 ng/ml) in a buffer containing calcium ions. The "+" means that the reagent was used. After incubation the mixture was analyzed by light microscopy and agglutination was only detected under condition 1 when SP-A was added. The experiments were repeated three times.