|
Condition
|
Reagent
|
Agglutination
|
|---|
| |
SP-A
|
SP-D
|
Goat-anti-human-SP-A antibody
|
Anti-goat antibody
| |
|---|
|
1
|
+
|
-
|
+
|
+
|
+
|
|
2
|
-
|
+
|
+
|
+
|
-
|
|
3
|
+
|
-
|
-
|
+
|
-
|
|
4
|
+
|
-
|
+
|
-
|
-
|
|
5
|
-
|
-
|
+
|
+
|
-
|
- The table shows five different conditions of the bead-assay. The beads were incubated alternatively with anti-goat antibody, goat-anti-human antibody, SP-A (1 ng recombinant human standard, final concentration 100 ng/ml) or SP-D (1 ng recombinant human standard, final concentration 100 ng/ml) in a buffer containing calcium ions. The "+" means that the reagent was used. After incubation the mixture was analyzed by light microscopy and agglutination was only detected under condition 1 when SP-A was added. The experiments were repeated three times.
