Figure 4From: S100A7/psoriasin expression in the human lung: unchanged in patients with COPD, but upregulated upon positive S. aureusdetection S100A7/psoriasin exhibits both, nuclear and perinuclear localization. (A) Endogenous S100A/psoriasin was visualized in A549 cells by confocal microscopy using the monoclonal Psoriasin/HID5/S100A7 antibody (Imgenex, clone 47C1068). (B and E) Nuclei were visualized with Toto-3 iodide (Molecular Probes). (D) Negative control was included by using mouse immunoglobulin isotype control antibody (IgG1). (C and F) Overlap between the S100A7/psoriasin staining or control IgG1 staining and nuclei staining, respectively, are shown in the merge panels. Bars: 10 μm. The results shown are representative of at least three independent experiments.Back to article page