Canonical Pathway Analysis of Serum IgE in CD4+ Lymphocytes. Figures A-C represent the results of Canonical Pathway Analyses using Ingenuity Pathway Analysis ([IPA], Ingenuity Systems®, http://www.ingenuity.com) software in all subjects, males, and females respectively. The significance of the association between the our data set and identified pathways from the Ingenuity Pathway Analysis library of canonical pathways was measured as a ratio of the number of genes from our dataset that map to the pathway divided by the total number of molecules that exist within the canonical pathway (presented as a line plot). Fischer's exact tests were used to calculate p-values (presented as -log10 p values in a bar graph). In all subjects 763 transcripts demonstrated nominally significant (p < 0.05) correlation with total serum IgE. Of these 763 transcripts, 652 (85%) mapped to a unique HUGO gene id and were used for IPA canonical pathway analysis. Six pathways were nominally significant using a Fisher's exact test and are listed in Figure 2A. Among males, 900 transcripts were correlated with total serum IgE, 873 (97%) mapped to a unique HUGO gene id, and thirteen pathways were significant (Figure 2B). Among females, 158 transcripts were correlated with total serum IgE, 154 (97%) mapped to unique HUGO gene ids, and six pathways were significant (Figure 2C).