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Table 1 List of the primer sequences as used for the RT-PCR and ISH experiments.

From: Expression of taste receptors in Solitary Chemosensory Cells of rodent airways

Gene UniGene ID Primer Sequence primer 5'-3' Annealing T °C
α-gustducin Rn.10456 Forward CTG CTC TGA CGA TCT ATC TC 57
   Reverse GGT CAC TTA CAG CTC ACT TC  
Tas1R1 Rn.92309 Forward CGG TTC ACT GTT GAG GAG AT 57.4
   Reverse CCT GAA GAA CAC TCT AGC CA  
Tas1R2 Rn.222086 Forward CAG TTC TGC ATA ACC TCA CG 55.5
   Reverse CTT GTA GGA CCA CAT GGA AC  
Tas1R3 Rn.81025 Forward AGT TGC TAC GCC AAG TGA AC 56.5
   Reverse AGG TGA AGT CAT CTG GAT GC  
Tas2R119 Rn.48782 Forward GTC ATT GTC GTT GTC CAT GC 60
   Reverse CTT CTG AGC AGG ATG TCT TG  
Tas2R121 Rn.48786 Forward TTA GTC TCT GGC TTG CCA CC 60.9
   Reverse AGA GTA AGA GGA AGG AGA CC  
Tas2R107 Rn.48784 Forward CAT TCT CAT TGG CTT GGC GA 55.2
   Reverse TTA AGT GCT GCA GTG CCT TC  
Tas2R13 Rn.48787 Forward TAG TCA CTT CAG CCT CTG GT 58.3
   Reverse TAG AGC AAG AGG AAG GAG AC  
Tas2R123 Rn.48792 Forward CAT GGA CTG GCT CAA GAG GA 60.2
   Reverse CTA AGA CAA GGC AGC ACA GA  
Tas2R105 Rn.48788 Forward GCC AAG AAC AAG AAG CTC TC 53.9
   Reverse GGA TAG ACG GAT GCA GTT GT  
Tas2R134 Rn.143008 Forward GTG ACA TGA TTG TGG CTT GC 55.2
   Reverse CGC CTC TTG TCT TGT GAT CT  
Tas2R126 Rn.48794 Forward CCT CAG ACA TGA TCC TCC TC 59
   Reverse GTG CCT CGG AAC TTG AGA TT  
GAPDH Rn.91450 Forward ACT GGC GTC TTC ACC ACC AT 61
   Reverse ATC CAC AGT CTT CTG GGT GG