Expression of taste receptors in Solitary Chemosensory Cells of rodent airways

BMC Pulmonary Medicine

Table 1 List of the primer sequences as used for the RT-PCR and ISH experiments.

Gene	UniGene ID	Primer	Sequence primer 5'-3'	Annealing T °C
α-gustducin	Rn.10456	Forward	CTG CTC TGA CGA TCT ATC TC	57
		Reverse	GGT CAC TTA CAG CTC ACT TC
Tas1R1	Rn.92309	Forward	CGG TTC ACT GTT GAG GAG AT	57.4
		Reverse	CCT GAA GAA CAC TCT AGC CA
Tas1R2	Rn.222086	Forward	CAG TTC TGC ATA ACC TCA CG	55.5
		Reverse	CTT GTA GGA CCA CAT GGA AC
Tas1R3	Rn.81025	Forward	AGT TGC TAC GCC AAG TGA AC	56.5
		Reverse	AGG TGA AGT CAT CTG GAT GC
Tas2R119	Rn.48782	Forward	GTC ATT GTC GTT GTC CAT GC	60
		Reverse	CTT CTG AGC AGG ATG TCT TG
Tas2R121	Rn.48786	Forward	TTA GTC TCT GGC TTG CCA CC	60.9
		Reverse	AGA GTA AGA GGA AGG AGA CC
Tas2R107	Rn.48784	Forward	CAT TCT CAT TGG CTT GGC GA	55.2
		Reverse	TTA AGT GCT GCA GTG CCT TC
Tas2R13	Rn.48787	Forward	TAG TCA CTT CAG CCT CTG GT	58.3
		Reverse	TAG AGC AAG AGG AAG GAG AC
Tas2R123	Rn.48792	Forward	CAT GGA CTG GCT CAA GAG GA	60.2
		Reverse	CTA AGA CAA GGC AGC ACA GA
Tas2R105	Rn.48788	Forward	GCC AAG AAC AAG AAG CTC TC	53.9
		Reverse	GGA TAG ACG GAT GCA GTT GT
Tas2R134	Rn.143008	Forward	GTG ACA TGA TTG TGG CTT GC	55.2
		Reverse	CGC CTC TTG TCT TGT GAT CT
Tas2R126	Rn.48794	Forward	CCT CAG ACA TGA TCC TCC TC	59
		Reverse	GTG CCT CGG AAC TTG AGA TT
GAPDH	Rn.91450	Forward	ACT GGC GTC TTC ACC ACC AT	61
		Reverse	ATC CAC AGT CTT CTG GGT GG

ISSN: 1471-2466