Figure 2

Established parameters of mouse bleomycin-induced lung injury and fibrosis: lung histological score, collagen and tissue volume density. Mice were treated with bleomycin (black columns) or saline (white columns) as in Fig. 1 and were sacrificed at early and late time-points. Lungs were sectioned for histologic analyses (left lungs) or homogenized for collagen measurement (right lungs). *P < 0.05, **P < 0.01, and ***P < 0.001 compared with saline-treated controls. Columns, mean; bars, SD. TVD, tissue volume density.