Figure 2

NPSR1 protein and mRNA expression in transient transfected HEK-293 cells. Transiently NPSR1-A or NPSR1-B overexpressing HEK-293 cells, with or without NPS stimulation, were stained with an N-terminal monoclonal antibody together with a FITC conjugated secondary antibody, and the fluorescence intensity was measured. The histogram plot (a) shows that the intensity, representing the amount of receptors expressed on the cell membrane, and the transfection efficiency, represented by the number of counts, is the same between the two isoforms. From the same experiment NPSR1 mRNA expression was measured showing that the level of NPSR1-A and -B mRNA is similar between the respective overexpressing cells as well (b). Histogram plot (c) illustrates the intensity of the two isoforms after 2 and 6 hours of NPS stimulation.