Figure 5

cAMP and Ca ²⁺ response downstream NPS-NPSR1-A and -B signalling. Intracellular second messenger response was measured on transiently NPSR1-A or -B overexpressing HEK293 cells stimulated with NPS. The cAMP response (a) was measured in a concentration-response way showing a greater increase of cAMP in NPS-NPSR1-A stimulated cells. In the Ca²⁺ experiment (b) a fixed dose of 1μM NPS was used and intracellular Ca²⁺ accumulation was measured before and after injection (t = 5 s) of NPS. The Ca²⁺ also illustrates an increased response in the NPSR1-A overexpressing cells compared to NPSR1-B. cAMP data is shown as mean ± SEM and * indicates significant (p < 0.05) difference between NPSR1-A and -B. The Ca²⁺ plot illustrates data from a representative experiment.