Figure 2

Intracellular localization of proSP-C ^WT and proSP-C ^A116D forms in stabile MLE-12 cells. Immunofluorescent analysis with an antibody against (A) EEA1 (red) and (B) syntaxin 2 (red) shows that while proSP-C^WT localized with syntaxin 2, a protein found within lamellar bodies as surfactant secretory vesicles, colocalization of proSP-C^A116D with syntaxin 2 was merely absent. ProSP-CA116D instead localized with in EEA1 which did not show any colocalization with proSP-C^WT. Nuclei are stained with DAPI (blue).