Figure 5

Intracellular lipid content and lipid secretion of MLE-12 cells expressing SP-C ^WT or SP-C ^A116D. (A) Intracellular lipid content of cells stably expressing WT SP-C or A116D mutant were quantified by mass spectrometry. Untreated cells (-) or cells treated with 10 μM methylprednisolone (+Met) or hydroxychloroquine (+Hyd) for 24 hours prior to sample isolation. Values were calculated as % of the mean of the untreated WT values. (B) After removal of detached cells, the lipids in the cell supernatant were analyzed and presented as in (A). The graphs show relative amounts of phosphatidylcholine and lyso-phosphatidylcholine. Only significant p-values are depicted.