Skip to main content

Table 1 Phospholipid profile of transfected MLE-12 cells expressing mutant SP-CA116D

From: The surfactant protein C mutation A116D alters cellular processing, stress tolerance, surfactant lipid composition, and immune cell activation

Ā 

Cell lysate

Supernatant

Ā 

WT

A116D

P

WT

A116D

P

Total phospholipids

(nmol/mg protein)

152.80 Ā± 9.6

126.50 Ā± 10.6

0.0334

20.57 Ā± 3.8

12.90 Ā± 6.2

0.0259

Phospholipid classes (% of total PL)

Ā Ā Ā Ā Ā 

Phosphatidylcholine

57.80 Ā± 1.0

52.80 Ā± 0.2

< 0.001

44.70 Ā± 0.1

38.73 Ā± 0.6

< 0.001

Lyso-Phosphatidylcholine

0.60 Ā± 0.1

0.90 Ā± 0.2

0.0171

5.90 Ā± 0.4

16.78 Ā± 1.3

0.001

Phosphatidylglycerol

0.30 Ā± 0.0

0.30 Ā± 0.0

ns

0.20 Ā± 0.1

0.10 Ā± 0.1

ns

Sphingomyelin

6.20 Ā± 0.3

6.90 Ā± 0.3

0.0061

8.03 Ā± 0.2

11.14 Ā± 0.2

< 0.0001

Ceramide

1.80 Ā± 0.1

1.60 Ā± 0.1

0.0133

3.30 Ā± 0.3

2.15 Ā± 0.2

0.0034

Glucosyl-Ceramide

0.10 Ā± 0.0

0.20 Ā± 0.0

ns

0.08 Ā± 0.0

0.09 Ā± 0.0

ns

Phosphatidylethanolamine

11.20 Ā± 0.5

14.50 Ā± 0.1

< 0.001

6.54 Ā± 0.2

6.53 Ā± 0.1

ns

Phosphatidylserine

6.50 Ā± 0.1

7.70 Ā± 0.3

< 0.001

11.71 Ā± 0.8

8.57 Ā± 0.1

0.0028

  1. Data are means Ā± standard deviation from three independent experiments each performed in duplicate. ns = not significant