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Figure 4 | BMC Pulmonary Medicine

Figure 4

From: Neutrophil necrosis and annexin 1 degradation associated with airway inflammation in lung transplant recipients with cystic fibrosis

Figure 4

Morphological and flow cytometric analysis of cultured peripheral blood neutrophils from healthy subject. Neutrophils were cultured in vehicle medium for 0, 5 h, 15 h, 22 hr, 39 h and 46 h in RPMI culture media and cell morphology (A) and flow cytometry (B) were analyzed. Flow cytometric analysis could not be obtained for 46-h cultured cells because of clumping cells. Graphs represent annexin V-FITC and PI labeling. The lower left (LL) quadrant shows the percentage of viable cells. The lower right (LR) quadrant represents the percentage of early apoptotic cells labeled by annexin V-FITC. The upper left (UL) quadrant represents the percentage of dead cells labeled by PI. The upper right (UR) quadrant contains the percentage of advanced apoptotic and necrotic cells labeled by both annexin V-FITC and PI. The results are representative of 10 experiments. Morphological images were obtained by preparing cells on the cytospin slide, stained with Diff-Quik Stain and images photographed (Olympus BX60, 40X).

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