Schematic outline of the animal experiments. Two groups, resembling eosinophilic (A) and neutrophilic asthma (B), were subjected to sensitization via i.p. injection and challenge through inhalation of ovalbumin (OVA). For the neutrophilic asthma model, animals were additionally challenged with lipopolysaccharide (LPS). A third group of animals in the neutrophilic asthma group, received steroid (GC) treatment 1 h prior challenge and lung mechanic assessment. As controls a final fourth group, received only vehicle (PBS) treatment during inhalation. Lung function testing was performed for all groups at day 17 followed by BAL fluid collection, differential cell count and proteomic analysis.