Lung Mechanics: Airway responsiveness was evaluated using forced oscillation technique (FOT) [Prime 2 perturbation, resp. system impedance (Z
) measurements]. (A,B) Measurements of methacholine (MCh) induced tissue damping (G, A) and elastance (H, B). The maximum MCh response (3 mg/kg) was measured in controls (PBS), OVA/OVA challenged group, OVA/LPS challenged group and OVA/LPS challenged mice that received steroid treatment (OVA/LPS/GC). Values are indicated as mean ± SE.*p < 0.05 (C vs OVA/OVA and C vs OVA/LPS); #p < 0.05 (OVA/LPS vs OVA/LPS/GC); (B) p = 0.06 (C vs OVA/LPS); (C) Measurements of methacholine (MCh) induced Newtonian resistance (RN) for different MCh doses (mg/kg). Values are indicated as mean ± SE. ǂ,*,§: p < 0.05; §§: p < 0.01 (C vs OVA/LPS); §§§: p < 0.001 (C vs OVA/LPS); The control data have been published previously .