Table 3 Subset of T cell population to be analyzed
Antibody-kit | Clone | Fluorescence | Catalog number |
|---|---|---|---|
CD4-CD8- CD3 | CD4:HP2/6, CD8: 143–44, CD3: 33-2A3 | CD4: FITC CD8: PE CD3: PERCP | 4FI8PEI3PPI-50 T. IMMUNOSTEP |
CD11c | BU-15 | FITC | 11CF3-100 T IMMUNOSTEP |
CD14 | 47-3D6 | APC | 14A-100 T IMMUNOSTEP |
CD15 | MCS-1 | FITC | 15 F-100 T IMMUNOSTEP |
CD16 | 3G8 | PE | 16PE2-100 T IMMUNOSTEP |
CD45 | HL30 | CF BLUE | 45CFB2-100 T IMMUNOSTEP |
CD45 RA | HI100 | APC | 45RAA2-100 T IMMUNOSTEP |
CD 62 L | DREG 56 | APC-eFluor 780 | 47-0629 eBioscience |
Human regulatory T cell cocktail* | CD4: SK3, CD25: 2A3, CD127: HIL-7R-M21 | CD4: fitc, CD 25: PE-Cy7, CD127: Alexa fluor 647 | 560249 (BD Pharmigen) |