TNC synthesis in lung fibroblasts. A) TNC and α-sma gene expression in control and IPF fibroblasts, under different conditions; (Black) basal, (Light Gray) TNC treatment and (Black Gray) TGF-β1 treatment (at 4 and 24 h). An increase in basal and induced TNC synthesis by TGF-β1 at 24 h was observed in IPF fibroblasts. α-sma gene expression was induced by TNC and TGF-β1 at 4 h, although the effect of TNC was not significant. B) IPF fibroblasts after TGF-β1 treatment (24 h and 48 h) showed a progressive increase of TNC and α-sma synthesis.