Figure 4

Suppression of LPS induction of NADPH oxidases by TLR4 blockade in BEAS-2B cells. Cells were treated with 20 μM astragalin or 20 μg/ml OxPAPC and then stimulated with 2 μg/ml LPS for 8 h. Cell lysates were prepared for Western blot analysis with a primary antibody against phospho-PKCβ2, p22^phox, p47^phox, and eotaxin-1. β-Actin protein was used as an internal control. The bar graphs (mean ± SEM, n = 3-4) represent quantitative results of the upper bands obtained from a densitometer. Means not sharing a common superscript refer to significant different at P < 0.05.