Figure 5

Effects of astragalin on H ₂ O ₂ (A and B)- or LPS (C)-induced activation of MAPK in BEAS-2B cells. Cells were exposed to 20 μM H₂O₂ for 24 h or 2 μg/ml LPS for 8 h in the absence and presence of 1–20 μM astragalin. Cell lysates were prepared for Western blot analysis with a primary antibody against phospho-ERK, phospho-Akt, phospho-p38, and phospho-JNK. β-Actin protein was used as an internal control. The bar graphs (mean ± SEM, n = 3-4) represent quantitative results of the upper bands obtained from a densitometer. Means not sharing a common superscript refer to significant different at P < 0.05.