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Figure 5 | BMC Pulmonary Medicine

Figure 5

From: Monocyte interaction accelerates HCl-induced lung epithelial remodeling

Figure 5

Blockade of monocyte-epithelial cell interaction or PDGF receptor attenuated EMT profiles. Primary human SAEC were treated for 30 min with serum-free SAGM either alone (pH 7.4) or containing HCl (pH 4.0). The medium was replaced with complete SAGM at pH 7.4. The cells were then incubated for 30 min with tyrphostin A9, a specific inhibitor for PDGF receptor tyrosine kinase at 5 or 10 μM. Primary human monocytes were added onto the monolayer of the SEAC and cocultured for 48 h. Expression of EMT markers was detected by Western blots.

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