Figure 2

Representative lung tissue sections from healthy individuals and patients with usual interstitial pneumonia (UIP), stained for mTOR, ZEB1, and ROCK1. The mTOR, ZEB1 and ROCK1 expression was not detected in alveolar epithelial cells of control normal lung tissue (Figure 2A, E, I, immunohistochemical staining, brown color, 200× magnification). Staining was weak or absent in normal area of UIP lungs (Figure 2B, F, J, 400× magnification). Cells overlying fibroblastic foci showed weak staining, however mesenchymal cells of the fibroblastic foci did not show mTOR, ZEB1 and ROCK1 expression (Figure 2C, G, K; arrows denote location of fibroblastic focus, 400× magnification). Expression of mTOR, ZEB1 and ROCK1 was compared in same fibrotic area (Figure 2D, H, L, 400× magnification).