Figure 2
From: Sepsis-induced lung inflammation is modulated by insulin
Expression of TGF-β in the lung parenchyma after CLP. Non-diabetic, diabetic and insulin-treated diabetic rats were subjected to CLP or SHAM (false operated) surgery. After 6 hours, the lungs were washed, removed and processed. The expression of TGF-β was assessed by immunohistochemistry, positive staining in brown (diffused) and nuclei in blue (A) and morphometric analysis (B) of stained area in μm2 at 400x magnification. Ten random non-coincident microscopic fields were evaluated for each group, n = 7/group. Scale bar =20 μm. Data are presented as mean + -SEM. *p < 0.01.