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Table 1 16S rRNA PCR primers used in real-time PCR

From: Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR

Bacterium

Primer

Sequence(5’- 3’)

References

Streptococcus pneumoniae

F

ACG CAA CTG ACG AGT GTG AC

[18]

R

GAT CGC GAC ACC GAA CTA AT

Klebsiella pneumoniae

F

GAG GTC GGT GGT TCA AGT C

[19]

R

TCG CAG TAA AGA TGG TGG AG

Pseudomonas aeruginosa

F

ATG GAA ATG CTG AAA TTC GGC

[20]