Figure 1

N6022 caused a dose-dependent decrease in Penh and BALF eosinophils in OVA-sensitized and non-sensitized mice. Non-sensitized or OVA-sensitized mice were treated with N6022 or PBS vehicle given via a single i.v. dose at 24 h prior to MCh challenge. IpBr (0.9 mg/kg/dose) + Alb (5.2 mg/kg/dose) was administered as inhaled doses at 48 h, 24 h, and 1 h prior to MCh as a positive control for the model. Dosing at 24 h prior to MCh challenge occurred one hour prior to the last OVA challenge on study day 22. Penh was measured at baseline and after challenge with saline followed by increasing doses of MCh aerosol. BALF eosinophils were quantified via light microscopy. N6022 attenuated Penh (A) and BALF eosinophils (B) in OVA-sensitized mice. OVA sensitization significantly increased BALF eosinophils (C) and Penh (D vs. A, Vehicle). N6022 attenuated Penh in non-sensitized mice (D). Bars are the means ± SEM of 10 to 30 mice per group for (A), (B), and (C) and 10 mice per group for (D). *p < 0.05 for treatment vs. vehicle, One-way ANOVA, Dunnett’s for (A), (B), and (D). *p < 0.05 for OVA-sensitized vs. non-sensitized, two-tailed Student’s t-test for (C).