N6022 increased BALF nitrite. OVA-sensitized mice were treated with a single i.v. dose of either N6022 or PBS vehicle administered 24 h prior to MCh challenge which was one hour prior to the last OVA airway challenge. Non-sensitized mice treated with a single i.v. dose of PBS at 24 h prior to MCh challenge (one hour prior to OVA) also were assessed. Nitrite levels in BALF were measured via ozone chemiluminescence. Bars are the means ± SEM of 6 mice per group. *p < 0.05 for N6022 vs. vehicle, One-way ANOVA, Dunnett’s.