Figure 1From: Inflammatory and cytotoxic effects of acrolein, nicotine, acetylaldehyde and cigarette smoke extract on human nasal epithelial cells Immunocytochemistry for cytokeratin 5 in nasal epithelial cell cultures. Primary nasal epithelial cell cultures were stained with a rabbit anti-human antibody against cytokeratin 5 (1:100). The primary antibody was detected using a secondary antibody coupled to Alexafluor 568 (1:500). Nuclei were stained blue with DAPI (×40). Each quadrant represents DAPI staining alone (upper left), staining for cytokeratin 5 alone (upper right), and staining for both DAPI and cytokeratin 5 (lower left).Back to article page