Figure 2

IL-8 release from PNECs after 24 h treatment with PA LPS in the presence or absence of 30 μ M acrolein for 1 h or 4 h, 50 μ M nicotine or 50 μ M acetylaldehyde for 4 h or 24 h, and 5% CSE for 4 h or 24 h. PNEC cultures were treated with increasing concentrations of LPS for 24 h ± pretreatment with acrolein, nicotine or acetylaldehyde (n = 4). Separate cultures were treated with 5% CSE for 4 h or 24 h (n = 4). Data are displayed as median ± IQR. * p < 0.05. The control IL-8 concentration was 1310 ± 223 pg/ml.