Figure 6

Effect of acrolein, nicotine and acetylaldehyde on NF-κB expression in PNEC cultures determined using western blotting. Western blot of phosho-NF-κB and IκB-α protein expression in PNECs (with beta-actin loading controls). Blot A: lanes 1–3 represent treatment of PNECs with 0, 10, 30 μM acrolein (1 h); blot B: lanes 1–3: 0, 5, 50 μM nicotine (1 h); blot C: lanes 1–3 represent treatment of PNECs with 0, 10, 50 μM acetylaldehyde (1 h) and blot D lanes 1–3: media, 5% CSE, 50% CSE (1 h). Densitometry included for phosho-NF-κB: actin ratio (n = 5 for each group). Data are displayed as median ± IQR. *p < 0.05.