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Figure 5 | BMC Pulmonary Medicine

Figure 5

From: Transient vascular and long-term alveolar deficits following a hyperoxic injury to neonatal mouse lung

Figure 5

Pecam1 protein analysis. A. Immunohistochemistry for Pecam1. Brown staining indicates localization of Pecam1 in the lung of normoxic and hyperoxic mice at three time points (D5, D28 and D56). Mayer’s hematoxylin staining (dark blue) indicates nuclei. Blood vessels are marked with arrows. Scale bar = 100 μm. B. Western blot for Pecam1 (140 kDa) in the lung at three time points (D5, D28 and D56). Actb is used as the loading control (42 kDa). Norm – Normoxia, Hyp – Hyperoxia. C. Levels of Pecam1 per total protein (relative to Actb) according to the Western blot. Error bars represent SEM, n = 3-4. Significant difference is marked with two asterisks (P < 0.01, n = 3-4).

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BMC Pulmonary Medicine

ISSN: 1471-2466

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