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Table 3 The inhibitory effect of anti-CD137 blocking antibody (10 μg/mL) (a137), 10 -6M methylprednisolone (MP) alone and in combination on granzyme B, IFNγ and TNFα expression by CD28null(28-) and CD28 + (28+) CD8+ NKT-like cells and NK cells following stimulation of PBMC from COPD patients (mean ± SEM, mean of 8 experiments) compared with control antibody

From: Targeting peripheral blood pro-inflammatory cytotoxic lymphocytes by inhibiting CD137 expression: novel potential treatment for COPD

 

Granzyme B

IFNγ

TNFα

 

CD8 + NKT-like

NK

CD8 + NKT-like

NK

CD8 + NKT-like

NK

 

28-

28+

 

28-

28+

 

28-

28-

 

a137

21 ± 2*

14 ± 2

14 ± 2

26 ± 3*

20 ± 3

22 ± 3

30* ± 4

23* ± 4

24 ± 3

MP

1 ± 1

3 ± 2

2 ± 2

2 ± 1

5 ± 6

43 ± 3^

2 ± 1

2 ± 1

47 ± 9^

a137 + MP

22 ± 4#

17 ± 3#

15 ± 3#

28 ± 3#

25 ± 2#

66 ± 3

32 ± 4#

25 ± 4#

70 ± 7#

  1. *The percentage inhibition of granzyme B, IFNγ and TNFα by CD28- and CD28+ NKT-like and NK cells compared with control (same specimen) without treatment/s.
  2. The inhibitory effect of anti-CD137 was greater than for MP for granzyme B, IFNγ and TNFα in all NKT-like cell subsets (p < 0.05 for all).
  3. *The inhibitory effect of anti-CD137 was greater than for the CD28nullCD8+ NKT-like subset than for the CD28 + CD8+ NKT-like subset for granzyme B, IFNγ and TNFα (p < 0.05 for all). ^The inhibitory effect of MP on IFNγ and TNFα by NK cells was significantly greater than for Granzyme B (p < 0.05) and significantly greater than the effect of anti-CD137.
  4. #The combination of anti-CD137 and 10-6 M methylprednisolone resulted in the additive inhibition of granzyme B, IFNγ and TNFα expression in CD28null and CD28+ CD8+ NKT-like cells and NK cells.