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Table 3 Primers and PCR cycling conditions.

From: Hepatocyte and keratinocyte growth factors and their receptors in human lung emphysema

Primers Sequences Denaturation annealing Cycles PCR products
HGF: Forward
Reverse
5'-CAGAGGGACAAAGGAAAAGAA-3'
5'-GCAAGTGAATGGAAGTCCTTTA-3'
94°C, 15s 58°C, 60s 40 167 bp
KGF: Forward
Reverse
5'-GAACAAGGAAGGAAAACTCTATGCAA-3'
5'-AAGTGGGCTGTTTTTTGTTCTTTCT-3'
94°C, 15s 60°C, 60s 40 201 bp
HGF-R: Forward
Reverse
5'-GTTTACTTGTTGCAAGGGAGAAGACT-3'
5'-TAGGGTGCCAGCATTTTAGCA-3'
94°C, 15s 58°C, 60s 40 88 bp
KGF-R: Forward
Reverse
5'-TTAAGCAGGAGCATCGCATTG-3'
5'-AACATCCAGGTGGTACGTGTGAT-3'
94°C, 15s 60°C, 60s 40 151 bp
Ubiquitin-c: Forward
Reverse
5'-CACTTGGTCCTGCGCTTGA-3'
5'-TTTTTTGGGAATGCAACAACTTT-3'
94°C, 15s 60°C, 60s 40 105 bp