Table 3 Primers and PCR cycling conditions.
From: Hepatocyte and keratinocyte growth factors and their receptors in human lung emphysema
Primers | Sequences | Denaturation | annealing | Cycles | PCR products |
|---|---|---|---|---|---|
HGF: Forward Reverse | 5'-CAGAGGGACAAAGGAAAAGAA-3' 5'-GCAAGTGAATGGAAGTCCTTTA-3' | 94°C, 15s | 58°C, 60s | 40 | 167 bp |
KGF: Forward Reverse | 5'-GAACAAGGAAGGAAAACTCTATGCAA-3' 5'-AAGTGGGCTGTTTTTTGTTCTTTCT-3' | 94°C, 15s | 60°C, 60s | 40 | 201 bp |
HGF-R: Forward Reverse | 5'-GTTTACTTGTTGCAAGGGAGAAGACT-3' 5'-TAGGGTGCCAGCATTTTAGCA-3' | 94°C, 15s | 58°C, 60s | 40 | 88 bp |
KGF-R: Forward Reverse | 5'-TTAAGCAGGAGCATCGCATTG-3' 5'-AACATCCAGGTGGTACGTGTGAT-3' | 94°C, 15s | 60°C, 60s | 40 | 151 bp |
Ubiquitin-c: Forward Reverse | 5'-CACTTGGTCCTGCGCTTGA-3' 5'-TTTTTTGGGAATGCAACAACTTT-3' | 94°C, 15s | 60°C, 60s | 40 | 105 bp |