Table 1 Sequences of oligonucleotides used as forward and reverse primers for real-time RT-PCR
From: Spatial and temporal expression of surfactant proteins in hyperoxia-induced neonatal rat lung injury
Gene product | Forward primer | Reverse primer |
|---|---|---|
SP-A | 5'-TACCAGAGCAGGAGGCAACA-3' | 5'-CAATACTTGCAATGGCCTCGTT-3' |
SP-B | 5'-CCATCCCTCTGCCCTTCTG-3' | 5'-CACCCTTGGGAATCACAGCTT-3' |
SP-C | 5'-TCCCAGGAGCCAGTTTCG-3' | 5'-CACGATGAGAAGGCGTTTGA-3' |
SP-D | 5'-AAATCTTCAGGGCGGCAAA-3' | 5'-GGCCTGCCTGCACATCTC-3' |
CC10 | 5'-TTACAACATCAGCCCACATCTACA-3' | 5'-TGTGATGCCGATCTTCATGGT-3' |
β-actin | 5'-TTCAACACCCCAGCCATGT-3' | 5'-AGTGGTACGACCAGAGGCATACA-3' |