Table 2 Between Cell Culture Condition Comparisons of TH2 Cell Positivity for IL-4 and IFN-γ
Condition | IL-4 Positive (%) | Fold IL-4 MFI | IFNγ Positive (%) | Fold IFNγ MFI | |
|---|---|---|---|---|---|
Positive Control (CD3/CD28) | Mean | 25.5% | 22.47 | 7.7% | 7.43 |
SEM | 6.8% | 12.26 | 1.3% | 1.42 | |
p-value (Negative Control) | 0.003 | 0.03 | 0.05 | 0.05 | |
p-value (Isotype Control) | 0.11 | 0.18 | 0.35 | 0.24 | |
Anti-NKG2A Antibody (CD3/CD28 + anti-NKG2A) | Mean | 9.3% | 4.39 | 5.5% | 5.29 |
SEM | 4.8% | 1.81 | 1.3% | 1.50 | |
p-value (Positive Control) | 0.001 | 0.01 | 0.04 | 0.01 | |
p-value (Isotype Control) | 0.005 | 0.066 | 0.427 | 0.234 | |
Isotype Control (CD3/CD28 + IgG2a) | Mean | 23.7% | 7.81 | 4.9% | 6.50 |
SEM | 1.0% | 0.72 | 2.7% | 0.43 | |
p-value | - | - | - | - | |
Negative Control (Culture Medium Alone) | Mean | 6.7% | 2.76 | 4.8% | 4.83 |
SEM | 4.6% | 0.63 | 0.8% | 1.60 | |
p-value | - | - | - | - |