Table 1 Primers, PCR conditions and restriction enzymes used for genotyping
Polymorphism | Primer | PCR condition | Restriction enzyme |
|---|---|---|---|
rs 2077079 | AGCCACAAGTCCAGGAACAT ATGCCTAGCACAAAGCAGTG | 65°-55°C in -0.5°C steps, 20 cycles, 55°C 24 cycles | 3U BSI1 |
rs 1130866 | TGGGGGATTAGGGGTCAGTC CCATGGGTGGGCACAGGGG | 65°-55°C in -0.5°C steps, 20 cycles, 55°C 20 cycles | 1U TaaI |
rs2040349 | GACACTGGAGACGGAGGACT AAAGCCAGCTGATGCTCTTC | 65°-55°C in -0.5°C steps, 20 cycles, 55°C 20 cycles | 1U NIaIV |
rs 3024801 | CCTAACACTCCCACCCTGTG TCCTCCCCTCTCTCTTCCTC | 65°-55°C in -0.5°C steps, 20 cycles, 55°C 20 cycles | pool sequencing |
rs 3024809 | GCTACTCCGTCATCCTGCTC GCCCATACCTTTTCCCTGTC | 65°-55°C in -0.5°C steps, 20 cycles, 55°C 20 cycles | pool sequencing |