Table 1 Primer and TaqMan™ probes

Hypoxanthin-guanine-phosphoribosyl-transferase

F: 5'-TGGAAAGAATGTCTTGATTGTTGAAG-3'

R: 5'-ATCTTTGGATTATGCTGCTTGACC-3'

Probe: 5'(VIC)-ACACTGGCAAAAVAATGCAAACCTTGCT-(TAMRA)3'

β-Actin

F: 5'-TCATCACCATCGGCAACG-3'

R: 5'-TTCCTGATGTCCACGTCGC-3'

Probe: 5'(VIC)-CCTTCCTGGGCATGGAGTCCTGC-(TAMRA)3'

Interleukin 1 beta (IL-1β)

F: 5'-GGTTTCTGAAGCAGCCATGG-3'

R: 5'-GATTTGCAGCTGGATGCTCC-3'

Probe: 5'(FAM)-AAAGAGATGAAGTGCTGCACCCAAAACCTG-(TAMRA)3'

Interleukin 6 (IL-6)

F: 5'-GGGTAGGGAAGGCAGTAGCC-3'

R: 5'-GAATCCCTCTCCACAAGCG-3'

Probe: 5'(FAM)-CTTCAGTGGAGTCGCCTTCTCCCTAA-(TAMRA)3'

Interleukin 8 (IL-8)

F: 5'-TTCTGCAGCTCTCTGTGAGGC-3'

R: 5'-GGTGGAAAGGTGTGGAAGTC-3'

Probe: 5'(FAM)-TTCTGGCAAGAGTAAGTGCAGAACTTCGATG-(TAMRA)3'

Interleukin 10 (IL-10)

F: 5'-TTGGAGCTTGCTAAAGGCACT-3'

R: 5'-CGGCGCTGTCATCAATTTCT-3'

Probe: 5'(FAM)-CACCTCCTCCACGGCCTTGCTCTT-(TAMRA)3'

Surfactant protein B (SP-B)

F: 5'-TCC GCT GGT CGT TGA TCA C-3'

R: 5'-GTT TGC ACA GGC CCA AGT G-3'

Probe: 5'(FAM)-CAG AGC CAA ATG AAC CTG AAG GCC ATC-(TAMRA)3'

Surfactant protein C (SP-C)

F: 5'-CAC CTT CTC CAT TGG CTC TAG TG-3'

R: 5'-ATA CTC TGC GGA GAC ATC TTC ATG-3'

Probe: 5'(FAM)-TGA CTA CCA GCG GCT CCT GAT TGC C-(TAMRA)3'