Surfactant replacement and open lung concept – Comparison of two treatment strategies in an experimental model of neonatal ARDS

Table 3 Phospholipid and surfactant protein analyses of lavage specimen

group	n (piglets)	PL [μg/ml]	PPQ	LSA [% PL]	SP-B/PL [% (w/w)]	SP-C/PL [% (w/w)]	PC [%]	PG [%]	PS [%]	PI [%]
PPV _OLC	5	48.65 ± 7.99	0.08 ± 0.01	47.7 ± 4.4	13.71 ± 3.08	3.06 ± 0.28	73.84 ± 5.57	3.59 ± 1.82	3.97 ± 2.44	6.87 ± 2.37
mon SP-B	4	131.10 ± 59.69	0.33 ± 0.16	62.7 ± 5.3	4.78 ± 2.01**	1.23 ± 0.45**	81.87 ± 1.38*	7.27 ± 2.25*	0.00 ± 0.00**	3.87 ± 1.94
nat SF	4	176.03 ± 68.91**	0.37 ± 0.21*	63.6 ± 6.2*	6.19 ± 1.73**	1.06 ± 0.62***	81.19 ± 1.43*	8.44 ± 1.10**	0.00 ± 0.00**	3.52 ± 0.79
group	n (piglets)	PE [%]	SPH [%]	PUFA [%]	Usat FA [%]	TL 16:0 [%]	Tlfa 20:4 (AA) [%]		Tlfa 20:5 (EPA) [%]
PPV _OLC	5	7.94 ± 3.63	3.80 ± 1.42	19.94 ± 1.39	40.34 ± 1.41	44.78 ± 1.73	5.54 ± 0.55		0.16 ± 0.03
monSP-B	4	4.99 ± 1.21	2.02 ± 0.85	12.16 ± 3.76**	33.19 ± 3.27**	52.97 ± 3.78**	3.03 ± 0.71**		0.15 ± 0.02
nat SF	4	0.47 ± 0.18	2.11 ± 0.81	11.52 ± 3.09**	35.76 ± 1.78*	50.24 ± 2.17**	2.58 ± 0.82***		0.29 ± 0.06**

PPQ (phospholipid-to-protein ratio); Phospholipid classes are given as percent (w/w) of all phospholipids. LSA = large surfactant aggregates, given as percent (w/w) of all BALF phospholipids. PC (phosphatidylcholine); PG (phosphatidylglycerol); PS (phosphatidylserine); PI (phosphatidylinositol); PE (phosphatidylethanolamine); SPH (sphingomyelin); Fatty acids were dermined in the BAL total lipid fraction and are given as percent (w/w) of all fatty acids. PUFA (polyunsaturated fatty acids in the total lipid fraction); TL 16:0 (palmitic acid in the total lipid fraction). AA = Arachidonic acid, EPA = Eicospentaenoic acid
* p < 0.05 vs PPV_OLC; ** p < 0.01 vs PPV_OLC; ***p < 0.001 vs PPV_OLC
§ p < 0.05 vs mon SP-B; §§ p < 0.01 vs mon SP-B

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