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Figure 2 | BMC Pulmonary Medicine

Figure 2

From: Human eosinophil adhesion and degranulation stimulated with eotaxin and RANTES in vitro: Lack of interaction with nitric oxide

Figure 2

Lack of effect of N ω -nitro-L-arginine methyl ester (L-NAME) upon eotaxin- and RANTES-induced eosinophil adhesion to fibronectin-coated plates. Eosinophils (3.5 × 103 cells/well) were incubated with platelet-activating factor (PAF; 10-6M), D-NAME; 0.1 mM), L-NAME (0.1 mM), eotaxin (EOT; 100 ng/ml) or RANTES (RAN; 100 ng/ml) for 4 h (37°C, 5%CO2), and then allowed to adhere to fibronectin-coated wells for 30 min. Results are expressed as mean adhered cell percentages of total cell number ± SEM (n = 6). * P < 0.05 compared to control (CTRL).

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