Figure 3
Effect of L-NAME and CC-chemokines upon eosinophil expression of α4 and αM subunits. Eosinophils (3.0 × 105 cells) were incubated or not (CTRL) with platelet-activating factor (PAF; 10-6M), Nω-nitro-L-arginine methyl ester (L-NAME; 0.1 mM), eotaxin (EOT; 100 ng/ml) or RANTES (RAN; 100 ng/ml) for 4 h (37°C, 5%CO2), and then were labelled with anti-α 4 (VLA-4; Panel A) or anti-αM (Mac-1; Panel B) antibodies. The results are expressed as mean fluorescence ± SEM (n = 4).