Treatment | Optical density |
---|
CTRL | 0.39 ± 0.02 |
A23187 (5 μM) | 0.87 ± 0.04* |
D-NAME (0.1 mM) | 0.27 ± 0.01 |
L-NAME (0.1 mM) | 0.70 ± 0.04* |
Eotaxin (100 ng/ml) | 0.70 ± 0.03* |
RANTES (100 ng/ml) | 0.71 ± 0.05* |
Eotaxin+L-NAME | 0.77 ± 0.02* |
RANTES+L-NAME | 0.69 ± 0.04* |
- Eosinophils (4.5 × 105 cells) were incubated or not (CTRL) with Nω-nitro-L-arginine methyl ester (L-NAME; 0.1 mM), eotaxin (100 ng/ml) or RANTES (100 ng/ml) for 4 h (37°C, 5%CO2). After incubation the released peroxidase in 50 μl of supernatant was measured and expressed as mean ± SEM of optical density (n = 4). *P < 0.05 compared to control (CTRL).