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Table 2 Effect of L-NAME and CC-chemokines upon eosinophil peroxidase (EPO) release.

From: Human eosinophil adhesion and degranulation stimulated with eotaxin and RANTES in vitro: Lack of interaction with nitric oxide

Treatment Optical density
CTRL 0.39 ± 0.02
A23187 (5 μM) 0.87 ± 0.04*
D-NAME (0.1 mM) 0.27 ± 0.01
L-NAME (0.1 mM) 0.70 ± 0.04*
Eotaxin (100 ng/ml) 0.70 ± 0.03*
RANTES (100 ng/ml) 0.71 ± 0.05*
Eotaxin+L-NAME 0.77 ± 0.02*
RANTES+L-NAME 0.69 ± 0.04*
  1. Eosinophils (4.5 × 105 cells) were incubated or not (CTRL) with Nω-nitro-L-arginine methyl ester (L-NAME; 0.1 mM), eotaxin (100 ng/ml) or RANTES (100 ng/ml) for 4 h (37°C, 5%CO2). After incubation the released peroxidase in 50 μl of supernatant was measured and expressed as mean ± SEM of optical density (n = 4). *P < 0.05 compared to control (CTRL).