Table 2 Number of T-RF bands resolved in T-RFLP profiles generated from the culture-independent and culture-based approaches.
| Â | Â | Direct Molecular Analysis | Culture-derived Molecular Analysis | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Patient | Condition | DNA | RNA | Total T-RFs | CLED | Â | PYO | Â | CHOC | Â | BLOOD | Â | CEP | Â | SNA | Â | CNA | Â | Total T-RFs |
| Â | Â | Â | Â | Â | DNA | RNA | DNA | RNA | DNA | RNA | DNA | RNA | DNA | RNA | DNA | RNA | DNA | RNA | Â |
1 | CF | 23 | 23 | 32 | 3 | 3 | 8 | 7 | 8 | 7 | 3 | 3 | 0 | 0 | 0 | 0 | 3 | 3 | 19 |
2 | CF | 3 | 10 | 10 | 3 | 2 | 2 | 2 | NA | NA | 3 | 2 | 2 | 2 | 0 | 0 | 3 | 3 | 6 |
3 | CF | 3 | 5 | 6 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 0 | 0 | 2 | 2 | 2 |
4 | CF | 3 | 3 | 3 | 3 | 3 | 3 | 1 | 3 | 5 | 2 | 3 | 2 | 2 | 0 | 0 | 5 | 8 | 11 |
5 | CF | 15 | 18 | 21 | 2 | 2 | 2 | 2 | 4 | 4 | 3 | 6 | 0 | 0 | 0 | 0 | 3 | 2 | 8 |
6 | CF | 7 | 8 | 9 | 2 | 2 | 3 | 3 | 3 | 4 | 4 | 4 | 2 | 2 | 0 | 0 | 2 | 4 | 4 |
7 | CF | 6 | 7 | 7 | 3 | 3 | 2 | 2 | 2 | 2 | 5 | 4 | 0 | 0 | 0 | 0 | 2 | 2 | 6 |
8 | CF | 10 | 19 | 27 | 4 | 4 | 6 | 6 | 4 | 4 | 0 | 0 | 2 | 2 | 2 | 2 | 3 | 5 | 12 |
9 | COPD | 6 | 9 | 13 | NA | NA | NA | NA | 2 | 2 | 2 | 5 | NA | NA | NA | NA | NA | NA | 8 |
10 | COPD | 11 | 10 | 17 | NA | NA | NA | NA | 2 | 2 | 5 | 7 | NA | NA | NA | NA | NA | NA | 11 |
11 | COPD | 8 | 19 | 19 | NA | NA | NA | NA | 9 | 8 | 3 | 3 | NA | NA | NA | NA | NA | NA | 11 |
12 | COPD | 11 | 29 | 32 | NA | NA | NA | NA | 5 | 5 | 4 | 4 | NA | NA | NA | NA | NA | NA | 8 |