Inhalation therapy with the synthetic TIP-like peptide AP318 attenuates pulmonary inflammation in a porcine sepsis model

Table 1 Specific real-time PCR primers

PCR assay	Oligonucleotide sequence (5′-3′)	GenBank no.
IL-1β	S: ACCCTgCAgCTggAggAT	NM_214055
	A: CCTTTggAgTTTCCCAggA
IL-6	S: CCAATCTgggTTCAATCAggA	NM_214399
	A: gTggTggCTTTgTCTggATTC
	FL: TgTCgAggCTgTgCAgATTAgTACCA(--FL)
	CY5: (L670-)gCACTgATCCAgACCCTgAggCAA(--PH)
TNF-α	S: CCCAgAAggAAgAgTTTCCA	NM_214022
	A: CggCTTTgACATTggCTACA
	FL: ggCCCAAggACTCAgATCATCgTC(--FL)
	CY5: (L670-)CAAACCTCAgATAAgCCCgTCgC(--PH)
iNOS	S: gATggCACCATCATAggggAC	NM_001143690
	A: ggCACCCTgggAACTCAA
	FL: TGGAACACCCCAAATACGAGTGGTTCC(--FL)
	CY5: (L670-)GGAGCTGGAGCTGAAGTGGTACGCCC(--PH)
COX-2	S: CCCCTTCTgCCTgACgC	NM_214321
	A: CTCTgCTCTggTCgATTgAgg
	FL: TCTATCTTACTggAACATggCATCACCCA(--FL)
	CY5: (L670-)TTTgTTgAATCATTTAgCAggCAAATTgCT(--PH)
AREG	S: ATTATGCTGCTGGACTGGAC	NM_214376
	A: TCGCTACCAGAAGGCATTT
TNC	S: GAGACCTGACTGCTACAGAG	NM_214230
	A: CACAACGACTTCCTTGAGTG
PPIA	S: CTTTCACAgAATAATTCCAggATT	NM_214353
	A: ggACAAgATgCCAggACC
	FL. ATgCTTCAggATAAAATTCTCATCATCAAA(--FL)
	CY5: (L670-)TTCTCTCCATAgATggACTTgCCACCA(--PH)
sENACg	S: ACTTCACCCCCATCTTCCAC	XM_003357614
	A: TTCAGGCCAAACTCAGCTC

S: sense primer, A: anti-sense primer, FL: fluorescein, CY5: CY5-labelled. IL-1β, Sus scrofa interleukin 1 beta (IL1B), mRNA; IL-6, Sus scrofa interleukin 6 (interferon, beta 2) (IL6), mRNA; TNF-α, Sus scrofa tumor necrosis factor (TNF superfamily, member 2) (TNF), mRNA; iNOS, Sus scrofa nitric oxide synthase 2, inducible (NOS2), mRNA. COX-2, Sus scrofa prostaglandin G/H synthase-2 (PGHS-2), mRNA; AREG, Sus scrofa amphiregulin (AREG), mRNA; TNC, Sus scrofa tenascin C (TNC), mRNA; PPIA, Sus scrofa peptidylprolyl isomerase A (cyclophilin A) (PPIA), mRNA; sENACg, Sus scrofa sodium channel, non-voltage-gated 1, beta subunit (SCNN1B), transcript variant X1, mRNA.

ISSN: 1471-2466