Figure 2

Quantification of mRNA abundance and protein expression of SERCA2 in ASM cells from asthmatic and non-asthmatic subjects. (A) The relative expression of SERCA2a/b/c mRNA normalized to 18S rRNA was not significantly different (Student’s t-test) in asthma (n = 13) compared to normal (n = 10) ASM donors. (B) An example western blot showing total SERCA2 protein and β-actin immunoreactivities in ASM cells from four asthma (A) and three normal (N) donors. (C) Densitometry of total SERCA2 immunoreactivity from western blots shows that there is no significant difference (Student’s t-test) in SERCA2 protein expression (normalized to β-actin expression) in asthma (n = 10) compared to normal (n = 10) ASM cell donors. Symbol key: filled circles: GINA 1–3; open circles: GINA 4–5; filled squares: non-asthmatic donors. Non-parametric data (A) displayed as median (IQR) and analyzed using Mann–Whitney. Parametric data (C) displayed as mean (SEM) and analyzed by Student’s t-test.