Figure 3
From: Immune targeting of the pleural space by intercostal approach
Altering of the pleural space cellular environment. (A) The pleural space contains CD11b+F4/80+ macrophages in the steady state. By using ICAPS injection of clodronate liposomes i.pls., flushing the pleural space with saline, or injection of talcum i.pls. leads to the removal of CD11b+F4/80+ macrophages 1 d after the procedure. A representative analysis is shown. (B) Total cell numbers significantly increase after injection of clodronate liposomes, and significantly decrease after flushing the pleural space or injection of talcum into the pleural space. The percentage (C) and enumeration (D) of CD11b+F4/80+ pleural space macrophages reveal their deletion within 1 day (n = 5; data shown in means ± S.D.; ***p < 0.001).