Figure 5
From: Immune targeting of the pleural space by intercostal approach
B1a cells evacuate the pleural space upon airway inflammation into the lungs. (A) Gating strategy of pleural space B1a B cells in the steady state. A representative analysis is shown. (B) Enumeration of B1a B cells in steady state, and 1, 2, and 3 days after LPS (20 μg/50 μl) i.n. challenge in the pleural space, the lungs and the blood in wt mice. (C) Enumeration of B1a B cells in the pleural space and lungs 1 d after LPS (20 μg/50 μl) i.n. challenge with or without flushing of the pleural space. (D) Enumeration of B1a B cells during steady state and 1 d after LPS (20 μg/50 μl) i.n. challenge in lymph nodes (LN), spleen, bone marrow (BM), and blood. (n = 5-8; data are shown in means ± S.D.; *p < 0.05; **p < 0.01; ***p < 0.001).