Figure 6
Change in cell density and diameter using FCFM according to the fluorophore (acriflavine or methylene blue) during the different stages of the malignant transformation of the hamster cheek pouch. A Median cell density (/mm2) calculated from imaging with acriflavine or methylene blue according to histological diagnosis. The data are plotted as median with range. Cell density was significantly higher in acriflavine-aided FCFM compared with methylene blue imaging. Respectively, it was 423 vs. 324 cells/mm2 in the hyperplastic stage (p = 0.006), 1093 vs. 459 cells/mm2 in the mild dysplastic stage (p = 0.006), 2574 vs. 694 cells/mm2 in the moderate dysplastic stage (p < 0.001), and 3163 vs. 2055 cells/mm2 in the severe dysplastic stage (p = 0.02). B. Median diameter (μm) measured using acriflavine or methylene blue-aided FCFM according to histological diagnosis. The data are plotted as median with range. Using acriflavine-aided FCFM, the diameter decreased during the different stages from hyperplasia to high-grade lesions (p = 0.003 between hyperplasia and mild dysplasia, p < 0.001 between mild and moderate dysplasia, p = 0.001 between moderate and severe dysplasia, p = 0.002 between moderate dysplasia and severe dysplasia/carcinoma).