Figure 4
Analysis of IL-6 levels in DMBT1+ and DMBT1- cells. IL-6 levels were determined by ELISA in cell culture supernatants of DMBT1+ and DMBT1- cells (A) without stimulation, (B-D) after stimulation with the respective agents. In the absence of inflammatory stimuli, DMBT1 had no significant effect on IL-6 levels. Compared to DMBT1- cells DMBT1+ cells showed significant suppression of IL-6 production after stimulation with the pro-inflammatory stimuli (TNFα, LPS, and PMA). The means of 5–14 cell culture wells are shown. Error bars represent standard error of the mean. Statistical significance has been evaluated using Mann–Whitney U-tests because the data lacked normal distribution.