Table 1 Technical validation of the EL-NE assay
From: Serological assessment of neutrophil elastase activity on elastin during lung ECM remodeling
Technical validation step | EL-NE |
|---|---|
Detection range | 0.90 nM – 119 nM |
Lower limit of quantification | 1.9 nM |
Intra-assay variation | 7% |
Inter-assay variation | 11% |
Dilution range of serum samples | 1:2 (recommended) |
Dilution range of plasma samples | 1:2 (recommended) |
Dilution recovery in serum | 104% |
Dilution recovery in plasma (Heparin) | 86% |
Dilution recovery in plasma (Citrate) | 108% |
Freeze-thaw recovery in serum | 96% |
Freeze-thaw recovery in plasma (Heparin) | 87% |
Freeze-thaw recovery in plasma (Citrate) | 104% |
Analyte stability serum (24 h, 4°C/20°C) | 88%/77% |
Analyte stability plasma (Heparin) (24 h, 4°C/20°C) | 95%/111% |
Analyte stability plasma (Citrate) (24 h, 4°C/20°C) | 85%/77% |