Figure 6

Masson’s trichrome staining for fibrosis in lung tissue sections from C57BL/6 mice treated with radiation and silibinin. (A-F) 80 days after irradiation; (G-L) 200 days after irradiation; (A, D, G, J) Lung tissue from sham-irradiated control; (B, E, H, K) Lung tissue from 13 Gy-irradiated mice; (C, F, I, L) Lung tissue from 13 Gy-irradiated mice with silibinin treatment. Masson’s trichome staining. Scale bars in low magnification present 300 μm. Scale bars in high magnification present 50 μm. (M) Percentage of fibrosis from lung tissue evaluated at 80 and 200 days following irradiation. The data are reported as means ± SEM (n = 5 per group). *p < 0.05 vs. sham-irradiated controls. ^#p < 0.05 vs. irradiated groups.