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Fig. 1 | BMC Pulmonary Medicine

Fig. 1

From: Protocadherin-1 is a glucocorticoid-responsive critical regulator of airway epithelial barrier function

Fig. 1

Expression of protocadherin-1 (PCDH1) protein in human bronchial epithelial (16HBE) cells. a Time course of PCDH1 protein expression in 16HBE cells. Cell lysates were harvested at the indicated time points. Western blot with anti-PCDH1 antibody showed two bands (150 and 170 kDa) corresponding to PCDH1 isoforms 1 and 2, respectively. β-Actin was used as an internal standard. b Graphs showing densitometric quantification of the PCDH1 isoform 1 or 2 bands on western blots, relative to β-actin. c Immunocytochemical analysis of PCDH1 (red) and cellular nuclei (blue) in 16HBE cells cultured for 24 and 72 h. The data represent the mean of three independent experiments

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